alexa Rv2820c of Beijing/W strains enhances Mycobacterium tub
ISSN: 2161-1068

Mycobacterial Diseases
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Research Article

Rv2820c of Beijing/W strains enhances Mycobacterium tuberculosis survival in human macrophages

J.T. Lam1, P.L. Ho1, X.H. Weng2, W.H. Zhang2, S. Chen2 and W.C. Yam1*

1Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pukfulam Road, Hong Kong Special Administrative Region, China

2Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Wulumuqi Road, Shanghai, China

Corresponding Author:
Yam WC
Department of Microbiology
The University of Hong Kong
University Pathology Building
Queen Mary Hospital Compound, Pukfulam Road
Hong Kong Special Administrative Region, China
Tel: (852) 2255 4821
Fax: (852) 2855 1241
E-mail: [email protected]

Received Date: October 28, 2011; Accepted Date: November 19, 2011; Published Date: November 25, 2011

Citation: Lam JT, Ho PL, Weng XH, Zhang WH, Chen S, et al. (2011) Rv2820c of Beijing/W strains enhances Mycobacterium tuberculosis survival in human macrophages. Mycobact Diseases 1:104. doi:10.4172/2161-1068.1000104

Copyright: © 2011 Lam JT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The ability to survive in human macrophages is a hallmark of the virulence of Mycobacterium tuberculosis. Although the intracellular parasitism is apparent, the molecular determinants behind are still largely unknown. The truncated Rv2820c of the Beijing/W strains of Mycobacterium tuberculosis was previously shown to enhance the survival of Mycobacterium smegmatis in the human macrophages. The enhanced intracellular survival, however, was not observed in the recombinant harboring the intact Rv2820c of the non-Beijing/W strains. In the current investigation, the role of the truncated Rv2820c in M. tuberculosis was examined using a ‘gain-of-function’ manner. The truncated Rv2820c was transformed into non-Beijing/W strains of M. tuberculosis and the resulting recombinants were used to infect the monocytic cell line THP-1. The ex vivo infection showed that the non-Beijing/W M. tuberculosis recombinants survived significantly better than the vector controls after ten days of infection (P < 0.05; independent samples t-test, two-tailed). Similar levels of interleukin-6, interleukin-10, and tumor necrosis factor-alpha were secreted from the macrophages infected with those non-Beijing/W recombinants. This study showed that the Rv2820c of the Beijing/W strains is capable of enhancing the M. tuberculosis survival in the human macrophages, but is unlikely to evoke a different profile of cytokine secretion from the infected macrophages. It suggests that the truncated Rv2820c may be another Beijing/W-specific virulence determinant.

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