Scoring System for Analyzing Vessel Development in Embryoid BodiesTimo Schinköthe1 and Annette Schmidt2,3*
- *Corresponding Author:
- Dr. Annette Schmidt
Ph.D, Department of Molecular and Cellular Sport Medicine
German Sport University, Cologne, Germany
E-mail: [email protected]
Received date: November 30, 2015; Accepted date: January 28, 2016; Published date: January 31, 2016
Citation: Schinköthe T, Schmidt A (2016) Scoring System for Analyzing Vessel Development in Embryoid Bodies. J Stem Cell Res Ther 6:325. doi:10.4172/2157-7633.1000325
Copyright: © 2016 Schinköthe T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Embryoid bodies (EB) derived from pluripotent embryonic stem cells (ES) are powerful tools for different purposes. One interesting aspect is vessel development and the use for simulating angiostatic effects of drugs. Unfortunately, it is difficult to compare the impacts of different drugs. Current methods allow the description of observations, but quantifying methods are missing. To overcome that gap, we have developed a score system to transfer microscopic observations into quantifiable values. The score system summarizes different vessel characteristics within few categories and allows the comparison of drug effects in view of time and dosage. We analysed score behaviour with respect to normal vessel development as well as under presence of angiotstatic drugs. The vessel score showed a time-dependent increase under normal conditions while under presence of an angiogenesis-inhibitor the score increase was slowed, and under angiogenesis enhancing conditions the score increase was accelerated. The presented vessel development score seems to be a helpful tool to transfer microscopic observations in EB vessel development into a quantifiable and comparable value.