alexa Screening and Identification of Extended Spectrum β -lactamase (ESBL) Pathogens in Urine Sample of UTI Patient ’ s | OMICS International
ISSN: 2329-9088

Tropical Medicine & Surgery
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Research Article

Screening and Identification of Extended Spectrum β -lactamase (ESBL) Pathogens in Urine Sample of UTI Patient ’ s

Jasmine Subashini and Krishnan Kannabiran*

School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India

*Corresponding Author:
Dr. K. Kannabiran
Biomolecules and Genetics Division
School of Biosciences and Technology
VIT University, Vellore-632014, India
Tel: +91 4162202200
Fax: +91 4162243092
E-mail: [email protected]

Received Date: February 18, 2013; Accepted Date: April 17, 2013; Published Date: April 21, 2013

Citation: Subashini J, Kannabiran K (2013) Screening and Identification of Extended Spectrum β-lactamase (ESBL) Pathogens in Urine Sample of UTI Patient’s. Trop Med Surg 1:120. doi: 10.4172/2329-9088.1000120

Copyright: © 2013 Subashini J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Extended Spectrum β-Lactamases (ESBL) exhibit most important resistant mechanism challenging broad spectrum of antibiotics, ESBL’s hamper current treatment strategies limiting therapeutic options. Hence, it is important to screen those organisms using standard techniques to fix the spread and outcome consequences. Aim and objective of our perspective cohort study was to isolate and to identify the resistant ESBL pathogens consecutive ESBL positive strains were recovered by Disc Diffusion Test (DDT) and 90% of the isolates were E. coli and Klebsiella pneumoniae. Further, antibiotic susceptibility assay against third line cephalosporins like cefotaxime (30 g), cefotaxime/clavulanic acid (30 μg/10 μg), ceftazidime (30 μg), ceftazidime/clavulanic acid (30 μg/10 μg), ampicillin (30 μg) and amikacin (30 μg) were tested by CLSI guidelines from NCCLS (National Committee for Clinical Laboratory Standards). Minimum Inhibitory Concentration (MIC) was determined against E-test ESBL strips containing cefotaxime (CT), cefotoxime/clavulanate (CTL), ceftazidime (TZ), ceftazidime/Clavulanate (TZL). E. coli (ATCC 25922) was used as negative control and (ATCC 700603) as a positive control strain. Controls were satisfactory in comparison to standard chart. Our study results unveil a very high spread of such super bugs in health centers. The empiric use of 3rd generation cephalosporins should be curtailed, as it is associated with increased risk of ESBL production. The necessity for continued antimicrobial resistance surveillance to prevent higher degree of resistance is justified in the light of our findings

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