alexa Screening and In-Vitro Analysis of Lactobacillus reuteri Strains for Short Chain Fatty Acids Production, Stability and Therapeutic Potentials in Colorectal Cancer | Abstract
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Screening and In-Vitro Analysis of Lactobacillus reuteri Strains for Short Chain Fatty Acids Production, Stability and Therapeutic Potentials in Colorectal Cancer

Kahouli I1,2, Malhotra M1, Tomaro-Duchesneau C1, Saha S1,5, Marinescu D1, Rodes LS1, Aloui-Jamali MA3,4 and Prakash S1,2*

1Biomedical Technology and Cell Therapy Research Laboratory-Departments of Biomedical Engineering, Physiology, and Artificial Cells and Organs Research Center. Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, H3A 2B4, Canada

2Department of Experimental Medicine, Faculty of Medicine, McGill University, 1110 Pine Avenue West, Montreal, Quebec, H3A 1A3, Canada

3Departments of Medicine and Oncology, Faculty of Medicine, McGill University, Gerald Bronfman Centre, Room 210, 546 Pine Avenue West, Montreal, Quebec, H2W 1S6, Canada

4Lady Davis Institute for Medical Research and Segal Cancer Centre, Sir Mortimer B. Davis-Jewish General Hospital, 3755 Côte Ste-Catherine Road, Montreal, Quebec, H3T 1E2, Canada

5Faculty of Dentistry, McGill University, 640 University Street, Montreal, Quebec, H3A 0C7, Canada

*Corresponding Author:
Prakash S
Biomedical Technology and Cell Therapy
Research Laboratory-Departments of Biomedical
Engineering Physiology, and Artificial
Cells and Organs Research Center
Faculty of Medicine, McGill University
3775 University Street, Montreal
Quebec, H3A 2B4, Canada
Tel: +1-514-398- 3676
Fax: +1-514-398-7461
E-mail: [email protected]

Received Date: November 27, 2014; Accepted Date: December 22, 2014; Published Date: December 28, 2014

Citation: Kahouli I, Malhotra M, Tomaro-Duchesneau C, Saha S, Marinescu D, et al. (2015) Screening and In-Vitro Analysis of Lactobacillus reuteri Strains for Short Chain Fatty Acids Production, Stability and Therapeutic Potentials in Colorectal Cancer. J Bioequiv Availab 7:039-050. doi: 10.4172/jbb.1000212

Copyright: © 2015 Kahouli I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The use of probiotics as preventive agents in colorectal cancer is widely reported in the literature. However, the bioactivity of specific bacterial strains is only partially understood. Here, we identified Lactobacillus reuteri strains with anti-proliferative activity against colorectal cancer cells. We investigated the bioavailability and the efficacy of short chain fatty acids secreted by distinct Lactobacillus reuteri strains on the inhibition of colorectal cancer cells growth. Five L. reuteri strains were screened based on the short chain fatty acids bio-production and anti-proliferative effects on Caco-2 colon cancer cells. The composition of probiotic short chain fatty acids in cell culture conditioned medium was used to prepare short chain fatty acid synthetic formulations that were compared with the L. reuteri cell culture conditioned media. Later, the bio-stability of the bacteria in a simulated intestinal fluid was determined. Results showed that the production of short chain fatty acids was strain-dependent. L. reuteri NCIMB -11951, -701359 and -702656 were the most potent in producing total short chain fatty acids (402.2 ± 23. 5, p < 0.05 compared with the rest of strains) and inhibiting Caco-2 (by 56.7 ± 1.6 % compared to untreated cells at 72 h, p < 0.001). Comparing the inhibitory effect of the probiotic cell culture conditioned medium and the corresponding short chain fatty acid synthetic formulation showed that the role and relevance of short chain fatty acid production in colorectal cancer cell growth suppression was strain-dependent. L. reuteri NCIMB -702656 and -701359 showed resistance in simulated intestinal fluid (104.6 ± 0.6 % and 105.7 ± 4.1 % of viability at 4 h, respectively) and produced high amounts of total short chain fatty acids (1245.49 ± 0.49 - 1391.58 ± 4.84 mg/ L at 24 h, respectively). Depending partly on short chain fatty acid bio-production, specific L. reuteri strains demonstrated growth inhibitory activity and may be considered as a potential chemopreventive agent against colorectal cancer.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

busin[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7