Screening of Antagonistic Marine Actinomycetes: Optimization of Process Parameters for the Production of Novel Antibiotic by Amycolatopsis Alba var. nov. DVR D4
Venkata Ratna Ravi Kumar Dasari*, Murali Yugandhar Nikku and Sri Rami Reddy Donthireddy
Center for Biotechnology, College of Engineering, Andhra University, Visakhapatnam- 530 003, India
- *Corresponding Author:
- Venkata Ratna Ravi Kumar Dasari
Center for Biotechnology, College of Engineering
Andhra University, Visakhapatnam- 530 003, India
E-mail: [email protected]
Received Date: November 07, 2011; Accepted Date: December 07, 2011; Published Date: December 09, 2011
Citation: Venkata Ratna Ravi Kumar D, Murali Yugandhar N, Sri Rami Reddy D (2011) Screening of Antagonistic Marine Actinomycetes: Optimization of Process Parameters for the Production of Novel Antibiotic by Amycolatopsis Alba var. nov. DVR D4. J Microbial Biochem Technol 3: 092-098. doi: 10.4172/1948-5948.1000058
Copyright: © 2011 Venkata Ratna Ravi Kumar D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Screening of six marine sediment samples near NTPC of the Visakhapatnam (India) Coast of Bay of Bengal resulted in the isolation of 72 isolates of actinomycetes. Among these, Amycolatopsis alba var. nov. DVR D4 showed broad antibacterial activity spectra against Gram-positive and Gram-negative bacteria; and produced antibacterial metabolite extracellulary under submerged fermentation conditions. The chemical and physical process parameters affecting the production of the antibiotic were optimized. The maximum antibiotic activity was obtained with the optimized production medium containing D-glucose, 2.0 %w/v; malt extract, 4.0 %w/v; yeast extract, 0.4 %w/v; dipotassium hydrogen phosphate, 0.5 %w/v; sodium chloride, 0.25 %w/v; zinc sulphate, 0.004 %w/v; calcium carbonate, 0.04 %w/v; with inoculum volume of 5.0 %v/v at 6.0 pH, 28 ° C incubation temperature, 220 rpm and for 96 h incubation.