Screening of Strong 1-Aminocyclopropane-1-Carboxylate Deaminase Producing Bacteria for Improving the Salinity Tolerance of Cowpea
Nguyen Huy Thuan*, Ho Viet Hieu and Nguyen Thanh Trung
Institute of Research and Development, Duy Tan University, K7/25 Quang Trung, Danang, Vietnam
- *Corresponding Author:
- Nguyen Huy Thuan
Institute of Research and Development
Duy Tan University, K7/25 Quang Trung, Danang, Vietnam
E-mail: [email protected]
Received date: January 14, 2016; Accepted date: March 11, 2016; Published date: March 21, 2016
Citation: Thuan NH, Hieu HV, Trung NT (2016) Screening of Strong 1-Aminocyclopropane-1-Carboxylate Deaminase Producing Bacteria for Improving the Salinity Tolerance of Cowpea. Appli Micro Open Access 2:111. doi: 10.4172/2471-9315.1000111
Copyright: © 2016 Thuan NH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aim: To isolate rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and evaluate the ability of selected bacteria for improving the growth of cowpea seedlings under salt stress conditions.
Methods: This study isolates salt-tolerant rhizobacteria which have strong ability to produce ACC deaminase and the phytohormone indol-3-acetic acid (IAA). Inoculation experiments with selected bacteria strains were used to verify the plant growth promoting activity of bacteria under salt stress conditions.
Result: Two isolates belong to Enterobacter cloacae and one isolate belongs to Pseudomonas sp. have been identified. Those rhizobacteria were found to be highly salt-tolerant at salinity level up to 10% NaCl. The selected bacterial strains were also capable to produce and secrete large amounts of ACC deaminase and the phytohormone IAA into the growth medium. Cowpea plants inoculated with ST3 strain revealed a significant increase in shoot length and shoot fresh weight over uninoculated control at the salinity level of 1.5% NaCl.
Conclusion: Three rhizobacterial strains belonging to the genera Enterobacter and Pseudomonas have been isolated. All three bacterial strains were identified as moderate halophiles and they can produce high levels of ACC deaminase and IAA. The strain Pseudomonas sp. ST3 showed the possible ability to promote the growth of cowpea under salt stress conditions.