Screening of Yellow Pigment Producing Bacterial Isolates from Various Eco-climatic Areas and Analysis of the Carotenoid Produced by the Isolate
Indra Arulselvi P*, Umamaheswari S, Ranandkumar Sharma G, Karthik C and Jayakrishna C
Plant and Microbial Biotechnology laboratory, Department of Biotechnology, Periyar University, Salem-11, Chennai
- *Corresponding Author:
- Indra Arulselvi P
Plant and Microbial Biotechnology laboratory
Department of Biotechnology
Periyar University, Salem, Chennai, India
Email: [email protected]
Received Date: December 31, 2013; Accepted Date: January 25, 2014; Published Date: January 31, 2014
Citation: Indra Arulselvi P, Umamaheswari S, Ranandkumar Sharma G, Karthik C, Jayakrishna C (2014) Screening of Yellow Pigment Producing Bacterial Isolates from Various Eco-climatic Areas and Analysis of the Carotenoid Produced by the Isolate. J Food Process Technol 5:292. doi:10.4172/2157-7110.1000292
Copyright: © 2014 Indra Arulselvi P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aim of the present was to isolate the microorganisms from different environment which is capable of producing high carotenoid pigments. Forty one soil samples were collected from different areas under different environmental and different climatic conditions. A total of 24 yellow pigmented colonies forming cocci shaped, gram positive bacteria were isolated. All the twenty four isolates were then subjected for carotenoid extraction using methanol as a solvent. High carotenoid producing isolates were confirmed by biochemical characterization and Mannitol salt growth. Of the 24 isolates tested, YCD3b showed highest production of carotenoid and it was confirmed through spectrophotometric analysis. Free Radical Scavenging Activity was studied by DPPH method. Isolate YCD3b displayed higher quantity of free radical scavenging activity compare to others isolates. Analysis of the peaks which were obtained at 450 nm indicated the presence of pigment, carotenoid.