alexa Screening, Production and Optimization of Extracellular
E-ISSN: 2252-5211

International Journal of Waste Resources
Open Access

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Research Article

Screening, Production and Optimization of Extracellular Laccase Enzyme from Pleurotus Ostreatus

Ambrin Farizah Babu*

Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Sholinganallur, Chennai, India

*Corresponding Author:
Ambrin Farizah Babu
Department of Biotechnology
Sathyabama University
Jeppiaar Nagar
Sholinganallur, Chennai, India
Tel: (65)-3615-8718
E-mail: [email protected]

Received date: January 05, 2016 Accepted date: January 21, 2016 Published date: January 27, 2016

Citation: Babu AF (2016) Screening, Production and Optimization of Extracellular Laccase Enzyme from Pleurotus ostreatus. Int J Waste Resources 6:199. doi:10.4172/2252-5211.1000199

Copyright: © 2016 Babu AF. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The enzyme laccase (p-diphenol: oxygenoxidoreductase; EC 1.10.3.2) is known to degrade many phenolic aromatic compounds. Many species of white rot fungi are well–known producers of the enzyme laccase. The first phase was the isolation of eight different strains of white rot fungi and screening them for laccase activity. Then highest laccase producing white rot fungal strains was identified. This strain Pleurotus ostreatus produced high amount of laccase compared to rest of the strains. In the second phase, the culture conditions for Pleurotus ostreatus were standardized to maximize the laccase production. The further steps involved the isolation, purification, and characterization of the enzyme laccase produced by Pleurotus ostreatus and in the final phase, the project deals with the textile industrial application of dyes and sludge treatment.

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