Selection, Expression and Purification of Single-Chain Fv Antibodies Against Non-structural Proteins of GB Virus C
Agne Valinciute-Jankauskiene* and Mykolas Mauricas
Immunology Department, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
- *Corresponding Author:
- Agne Valinciute-Jankauskiene
State Research Institute Centre for Innovative Medicine
E-mail: [email protected]
Received Date: February 23, 2015; Accepted Date: April 16, 2015; Published Date: April 23, 2015
Citation: Valinciute-Jankauskiene A, Mykolas Mauricas (2015) Selection, Expression and Purification of Single-Chain Fv Antibodies Against Non-structural Proteins of GB Virus C. J Antivir Antiretrovir 7:052-059. doi: 10.4172/jaa.1000119
Copyright: © 2015 Valinciute-Jankauskiene A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The GB virus C (GBV-C) exists worldwide, and GBV-C infection is common among healthy blood donors as well as among immunocompromised individuals. It has been shown that GBV-C may have a beneficial impact on HIV disease progression. The results of recent studies also demonstrated that GBV-C may increase survival of Ebola virus patients. However, there is no commercially available GBV-C detection system which would identify past infection. The aim of this study was to select and express functional single-chain, variable fragment (scFv) antibodies against GBV-C non-structural proteins. In order to achieve this aim, we expressed GBV-C non-structural proteins in E. coli and constructed a naïve scFv library. This study is the first to identify recombinant antibodies against GBV-C by using phage display. Six scFv that showed the highest affinity score by ELISA were successfully expressed. These recombinant scFv antibodies may provide a valuable tool for further study of GBV-C and contribute to the improvement of a rapid and accurate test for identification of GBV-C infection.