Sensitive Analysis of Off-flavor Compounds, Geosmin and 2-Methylisoborneol, in Water and Farmed Sturgeon by using Stir Bar Sorptive Extraction Coupled with Thermal Desorption and Gas Chromatography-Mass Spectrometry
Ruan ED*, Aalhus J and Juarez M
Lacombe Research Centre, Agriculture and Agri-Food Canada, 6000 C&E Trail, Lacombe, AB T4L 1W1, Canada
- *Corresponding Author:
- Eric Ruan
Lacombe Research Centre
Agriculture and Agri-Food Canada
6000 C&E Trail, Lacombe, AB T4L 1W1, Canada
E-mail: [email protected]
Received date: May 21, 2014; Accepted date: June 27, 2014; Published date: June 30, 2014
Citation: Ruan ED, Aalhus J, Juarez M (2014) Sensitive Analysis of Off-flavor Compounds, Geosmin and 2-Methylisoborneol, in Water and Farmed Sturgeon by using Stir Bar Sorptive Extraction Coupled with Thermal Desorption and Gas Chromatography-Mass Spectrometry. J Chromatograph Separat Techniq 5:228. doi:10.4172/2169-0316.1000228
Copyright: © 2014 Ruan ED, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The semi-volatile cyclic alcohols 2-methylisoborneol (2-MIB) and geosmin (GSM) impart muddy or musty flavors to water and fish products. A rapid quantitative analytical technique has been developed based on stir bar sorptive extraction (SBSE), coupled with thermal desorption and gas chromatography-mass spectrometry (TD-GCMS). SBSE is used to extract and concentrate the off-flavor compounds of GSM and 2-MIB in coating material polydimethylsiloxane (PDMS). The analytes are thermally released from SBSE and analyzed by GC-MS. Limits of detection (LOD) and limits of quantification (LOQ) of GSM and 2-MIB are 0.3 ng/L and 1 ng/L, based on the main fragment ions of m/z 112 and m/z 95, respectively. This methodology allows quantitative analysis of 2-MIB and GSM in both water and fish tissue successfully.