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Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Jinghui Wang, Mohammed AlMakhaita, Sibani Lisa Biswal* and Laura Segatori*

Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA

*Corresponding Author:
Sibani Lisa Biswal
Department of Chemical and Biomolecular Engineering
Rice University, Houston, TX 77005, USA
Tel: +1 (713) 348-6055
Fax: +1 (713) 348-5478
E-mail: [email protected]

Laura Segatori
Department of Chemical and Biomolecular Engineering
Rice University, Houston, TX 77005, USA
Tel: +1 (713) 348-3536
Fax: +1 (713) 348-5478
E-mail: [email protected]

Received Date: December 09, 2011; Accepted Date: January 03, 2012; Published Date: February 07, 2012

Citation: Wang J, AlMakhaita M, Biswal SL, Segatori L (2012) Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance. J Biosens Bioelectron 3:115. doi: 10.4172/2155-6210.1000115

Copyright: © 2012 Wang J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Currently available TNT sensors are characterized by high sensitivity, but low specificity, which limits the detection of TNT in dirty environments. We report here a TNT sensor designed to measure the displacement of a TNT-specific antibody by quartz crystal microbalance (QCM). This sensor combines high sensitivity of detection (0.1 ng/mL) with the ability to distinguish TNT from molecules with similar chemical properties. Particularly, the reliability of this method for the detection of TNT in dirty environments was investigated by using fertilizer solution and artificial seawater. Instead of measuring actual binding of TNT, the method described is based on the displacement of an anti-TNT antibody, which allows quantifying the concentration of TNT in solution with higher sensitivity. In addition, by utilizing the rate of antibody displacement, the detection time is significantly decreased from hours, which would be necessary to measure the frequency change at equilibrium, to minutes. A Langmuir kinetic model was used to describe the molecular interactions on the surface of the sensor and to establish a standard curve to estimate on-site TNT detection. In summary, QCM detection of anti-TNT antibody displacement provides a method for rapid detection of TNT with high sensitivity and specificity.

Keywords

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