alexa Sequence Variation and Recognition Specificity of the Avirulence Gene <em>AvrPiz-T</em> in <em>Magnaporthe Oryzae</em> Field Populations | OMICS International | Abstract
ISSN: 2165-8056

Fungal Genomics & Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Sequence Variation and Recognition Specificity of the Avirulence Gene AvrPiz-T in Magnaporthe Oryzae Field Populations

Chenxi Chen1,2#, Meilian Chen1#, Jinnan Hu2, Wenjing Zhang1, Zhenhui Zhong1, Yulin Jia3, Ludovic Allaux4, Elisabeth Fournier5, Didier Tharreau4, Guo-Liang Wang2,6, Zonghua Wang1, Wei-Chiang Shen7, Guodong Lu1, Baohua Wang1*, Thomas K. Mitchell2*
1Key Laboratory of Bio-pesticide and Chemistry Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
2Department of Plant Pathology, The Ohio State University, Columbus, OH, 43210, USA
3USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, AR, 72160, USA
4CIRAD, UMR 385 Biologie et Génétique des Interactions Plante-Parasite, F-34398 Montpellier, France
5INRA, UMR 385 Biologie et Génétique des Interactions Plante-Parasite, F-34398 Montpellier, France
6Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agronomy, Hunan Agricultural University, Hunan 410128, China
7Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan
#These authors contributed equally to this work
Corresponding Authors : Baohua Wang
Key Laboratory of Bio-pesticide and Chemistry Biology
Ministry of Education, Fujian Agriculture and Forestry University
Fuzhou, 350002, China
Tel: 86-591-86393212
E-mail: [email protected]
  Thomas Mitchell
Department of Plant Pathology
The Ohio State University
Columbus, OH, 43210, USA
Tel: (614) 917-9053
E-mail: [email protected]
Received May 24, 2014; Accepted July 25, 2014; Published July 28, 2014
Citation:Chen C, Chen M, Hu J, Zhang W, Zhong Z, wt al. (2014) Sequence Variation and Recognition Specificity of the Avirulence Gene AvrPiz-T in Magnaporthe Oryzae Field Populations. Fungal Genom Biol 4:113. doi:10.4172/2165-8056.1000113
Copyright: ©2014 Chen C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Magnaporthe oryzae, the rice blast pathogen, causes significant annual yield loss of rice worldwide. Currently, the most effective disease control approach is deployment of host resistance through introduction of resistance (R) genes into elite cultivars. The function of each R gene relies on the specific recognition of an avirulence (AVR) gene of the pathogen. However, the introduced resistance can be broken down within a few years, due to mutation of the AVR genes in the field populations. It is known from a few case studies that AVR mutation patterns are different from one to another. Therefore, knowledge of AVR genes sequence diversity serves as fundamental background in introducing new resistance to control rice blast. In this study, we focused on a newly identified AVR gene AvrPiz-t. We PCR amplified open reading frames (ORFs) of AvrPiz-t as well as promoter regions to detect size variation at this locus in 711 isolates of M. oryzae collected from 38 countries and regions. Through sequencing and Southern hybridization of the amplified locus, strains with polymorphisms in the ORF were classified into groups based on mutation type and site. Natural selection intensity on this gene was calculated and pathogenicity assays were applied to evaluate the association between AvrPiz-t ORF/promoter polymorphism and virulence. In conclusion, sequences at the AvrPiz-t locus were revealed to contain variations at both promoter and ORF regions. This locus is undergoing a relatively strong positive selection. The diversity in coding sequence and the insertions of transposable elements in the promoter region enable M. oryzae to evade recognition by the cognate Piz-t R gene in the host.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immunomicrobiol[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7