Sero-conversion of Avian Influenza Virus Subtype H9N2 in Non-Vaccinated Commercial Poultry FlocksNaseem Fawad1, Zahid Hussain1, Sohail Manzoor2*, Saad Rahman1, Najam-ul-Islam1, Muhammad Mukhtar1, Asgher Ali1 Halima Sadia1, Syed Abbas Ali3, Malik Muhammad Nawaz3, Fraz Munir Khan2, Farhan Afzal1 and Muhammad Asif1
- *Corresponding Author:
- Sohail Manzoor
Diagnostic Laboratories, North & South Punjab, Lahore, Pakistan
E-mail: [email protected]
Received date: November 16, 2016; Accepted date: December 02, 2016; Published date: December 15, 2016
Citation: Fawad N, Hussain Z, Manzoor M, Rahman S, Islam NU, et al. (2016) Sero-conversion of Avian Influenza Virus Subtype H9N2 in Non-Vaccinated Commercial Poultry Flocks. J Antivir Antiretrovir 8:136-138. doi: 10.4172/jaa.1000149
Copyright: © 2016 Fawad N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sero-conversion of pathogenic subtypes (H5, H7 and H9) of Avian Influenza Virus types was studied in commercial poultry farms in the Province of Punjab, Pakistan during the years 2015-16. Out of 6731 morbid tissues, only 11 samples (4 from broiler, 4 from broiler breeder and 3 from commercial layers farms) were found positive for Avian Influenza Virus (AIV) H9N2 type. Only one isolate was found positive for H9N2 subtype from 17994 tracheal swab samples. All the 37163 cloacal swab samples were found negative. The sero-analysis of 133438 blood sera samples showed sero-conversion in 66219 samples (49.64%) against H9 virus in the non-vaccinated poultry flocks, when tested with Heamagglutination Inhibition test (HI). Highest sero-conversion was recorded in broiler breeder (49.20%) followed by broilers (47.47%), layer (46.25%) and Native/Desi birds (45.59%). Highest HI titres were recorded in the flocks from Muzaffargarh, Rawalpindi, Toba Tek Singh, Sargodha, Gujrat, Faisalabad, Sheikhupura, Jhelum and Pakpattan while Bahawalpur, Gujranwala, Lahore, Jhang, Okara and Multan had relatively lower level of HI antibody titres. In conclusion only one type of AIV subtype H9N2 is prevailing in the province of the Punjab, Pakistan.