alexa Sero-diagnosis of Active Mycobacterium tuberculosis Dis
ISSN: 2161-1068

Mycobacterial Diseases
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Research Article

Sero-diagnosis of Active Mycobacterium tuberculosis Disease among HIV Coinfected Persons using Thymidylate Kinase based Antigen and Antibody Capture Enzyme Immuno-Assays

Misaki Wayengera1-3*, Ivan Mwebaza2, Johnson Welishe2, Cynthia Nakimuli2, David P Kateete2,3, Eddie Wampande2,3, Samuel Kirimunda2,3, Lois Bayigga2,3, Carol Musubika2,3, Peace Babirye2, Benon Asiimwe2,3 and Moses L Joloba2,3

1Department of Pathology, Unit of Genetics and Genomics, School of Biomedical Science, Makerere University College of Health Sciences, Kampala, Uganda

2Department of Immunology/Molecular Biology/Mycobacteriology, School of Biomedical Sciences, Makerere University College of Health Sciences, Uganda

3Department of Medical Microbiology, School of Biomedical Sciences, Makerere University College of Health Sciences, Kampala, Uganda

Corresponding Author:
Misaki Wayengera
Department of Pathology, Unit of Genetics and Genomics
School of Biomedical Science, Makerere University College of Health Sciences, Kampala, Uganda
Tel: +256782450610
E-mail: [email protected]

Received Date: May 14, 2017; Accepted Date: May 27, 2017; Published Date: May 31, 2017

Citation: Wayengera M, Mwebaza I, Welishe J, Nakimuli C, Kateete DP, et al. (2017) Sero-diagnosis of Active Mycobacterium tuberculosis Infections among HIV Co-infected Persons using Thymidylate Kinase based Antigen and Antibody Capture Enzyme Immuno-Assays. Mycobact Dis 7:241. doi:10.4172/2161-1068.1000241

Copyright: © 2017 Wayengera M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Clinical and laboratory diagnosis of Active Tuberculosis (ATB) and latent Mycobacterium Tuberculosis (M. tuberculosis) infections (LTBI) among people living with HIV/AIDS (PLWHA) presents formidable challenges. In the past, WHO issued an advisory against the use of existing TB sero-diagnostics? Emerging evidence, however, points to a precision of TB sero-diagnostics based on secretory rather than structural M. tuberculosis antigens. We hypothesized that secretory levels of M. tuberculosis thymidylate kinase (TMKmt) can designate ATBI from LTBI and no TB (NTB). Here, we report in-house validation studies of levels of TMKmt antigen (Ag) and host specific TMKmt antibody (Ab) amongst HIV +ve and HIV -ve participants.

Methods and Results: Direct TMKmt Ag and host specific IgG Ab detection EIAs were conducted on broadly consented, stored serum (N=281[Ag] vs. 214 [Ab] respective ) samples stratified as either HIV +ve or HIV–ve ATBI relative to LTBI and No TB. On one hand, UG-peptide 1 and its PAb-based EIAs accurately diagnosed ATB relative to LTBI and NTB among HIV +ve subjects {irrespectively: (a) Ag detection ATB=OD>0.490; 95% CI: 0.7446 to 0.8715 vs. LTBI=OD<0.490; 95% CI 0.4325 to 0.4829 vs. NTB=OD<0.26; 95% CI 0.1675 to 0.2567 and (b) TMKmt specific IgG detection ATB=OD>1.00; 95% CI 1.170 to 1.528 [HIV +ve] and 2.044 to 2.978 [HIV -ve] respectively vs. LTBI=OD<1.00; 95% CI 0.2690 to 0.6396 vs. NTB=OD<; 95% CI 0.1527 to 0.8751}. HIV -ve ATB presented with Ag levels greater than NTB and less than LTBI (i.e. ATB -ve=<0.490 ODs>0.26), but displayed better ant-TMKmt IgG responses (OD>2.00; 95% CI 2.044 to 2.978) relative to HIV +ve ATB (OD<1.600; 95% CI 1.170 to 1.528); suggesting a better control of M. tuberculosis-septicemia. On the other hand, UG-peptide 2 and its PAb-based EIAs did not demonstrate ATB diagnostic potential regardless of HIV sero-status, except towards designating NTB.

Conclusions: TMKmt Ab and Ag detecting EIAs based on UG-peptide 1 and its derivative PAb can accurately demarcate ATBI from LTBI and NTB among HIV +ve subjects.

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