alexa Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt
ISSN: 2329-888X

Advances in Dairy Research
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Research Article

Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt

Ahmed M El-Hady1, Mohamed Sayed-Ahmed2*, Mohamed E Saleh1 and Emad E Younis2

1Animal Health Research Institute, Dokki, Giza, Egypt

2Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Corresponding Author:
Mohamed Zakaria Sayed-Ahmed
Department of Internal Medicine and Infectious Diseases
Faculty of Veterinary Medicine
Mansoura University, Mansoura 35516, Egypt
Tel: +966-594-886878
Fax: +966-17-3216837
E-mail: [email protected]

Received Date: December 30, 2015; Accepted Date: March 09, 2016; Published Date: March 18, 2016

Citation: El-Hady AM, Ahmed MS, Saleh ME, Younis EE (2016) Seroprevalence and Molecular Epidemiology of Brucellosis in Cattle in Egypt. Adv Dairy Res 4:153. doi: 10.4172/2329-888X.1000153

Copyright: © 2016 El-Hady AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The aim of the present study was to evaluate of the Brucella vaccination program and immune response of vaccinated animals in Sharqyia Province. The study was applied on 710 lactating and non-lactating cows of some private farms and individual distributed animals on different districts in Sharqyia Province. Sera were collected from animals during routine diagnosis and control program. The results of screening tests Buffer acidified plate antigen test (BAPAT), Rose Bengal plate test (RBPT), Tube agglutination test (TAT), Complement fixation test (CFT), Rivanol test (RIVT) and indirect enzyme linked immunosorbent assay gave 124 and 176 seroreactive animals by incidence of 4.42% and 8.91% in private farms and individual cases respectively. 37 (29.8%) and 97 (55.1%) isolates of Brucella melitensis biovar 3 were recovered from 124 and 176 seroreactive animals respectively. In seroreactive cows, Brucella melitensis biovar 3 was isolated from 36% and PCR yielded expected products in 40%. In conclusion, more attention should be paid to the role of Brucella melitensis biovar 3 in brucellosis in cattle during the application of national program of Brucella control and eradication.


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