Seroprevalence of Toxoplasma Gondii in Immunocompetent and Immunocompromised Patients Using IgG - Modified Direct Agglutination Test (IgG MAT)
- *Corresponding Author:
- Sucilathangam Ganesan
Department of Microbiology
Tirunelveli Medical College
Tirunelveli, Tamil Nadu, India
E-mail: [email protected]
Received Date: November 08, 2011; Accepted Date: January 05, 2012; Published Date: January 09, 2012
Citation: Sucilathangam G, Palaniappan N, Sreekumar C, Anna T (2012) Seroprevalence of Toxoplasma Gondii in Immunocompetent and Immunocompromised Patients Using IgG - Modified Direct Agglutination Test (IgG MAT). J Medical Microbiol Diagnosis 1:102. doi: 10.4172/2161-0703.1000102
Copyright: © 2012 Sucilathangam G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background/Objective:The present study was conducted to assess the seroprevalence of Toxoplasma gondiiin and around Tirunelveli, Tamil Nadu, India by in-house IgG assay, using MAT.
Methods:Serum samples from 175 immunodeficient and 175 immunocompetent patients were collected at Tirunelveli District, Tamil Nadu from May 2006 to October 2007. They were subjected to in-house IgG assay using Modified Direct Agglutination test (MAT).
Results:Out of 350 patients tested by MAT, 39 patients (11.41%) had antibodies for toxoplasmosis with mean MAT titre of 53.85 ± 55.28 and the titre ranged from 1:20 to 1:160. Among the immunocompetent group of 175 patients, 18 patients (10.29%) had antibodies to toxoplasmosis whereas in immunodeficient group of 175 patients, 21 patients (12%) had antibodies for toxoplasmosis. The sensitivity, specificity of IgG MAT in detecting toxoplasmosis was 80 and 90 % respectively.
Statistical analysis: The results were analysed by using the statistical software SPSS.
Conclusions:The overall seroprevalence of toxoplasmosis in and around Tirunelveli District of Tamil Nadu was 11.41% based on IgG MAT. The study has proved that MAT is recommended as the most convenient method for serodiagnosis of toxoplasmosis and has the additional advantage of not requiring species-specific conjugates.