alexa Serum Complement Activity in Two Species of Divergent Central African Crocodiles | OMICS International | Abstract
ISSN: 2161-0983

Entomology, Ornithology & Herpetology: Current Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Serum Complement Activity in Two Species of Divergent Central African Crocodiles

Mark Merchant1*, Charles Determan1, Rodolfo Falconi1 and Matthew Shirley2

1McNeese State University, Department of Chemistry, Lake Charles, LA, USA

2University of Florida, Department Wildlife Ecology and Conservation, Gainesville, FL, USA

*Corresponding Author:
Mark Merchant, Ph.Dc
Professor of Biochemistry, McNeese State University
Lake Charles, LA, USA
Tel: (337) 475-5773
Fax: (337) 475-5950
E-mail: [email protected]

Received date: August 06, 2013; Accepted date: September 24, 2013; Published date: October 01, 2013

Citation: Merchant M, Determan C, Falconi R, Shirley M (2013) Serum Complement Activity in Two Species of Divergent Central African Crocodiles 2:110. doi: 10.4172/2161-0983.1000110

Copyright: © 2013 Merchant M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Serum complement in the serum of two divergent Central African crocodiles, the African dwarf crocodile (Osteolaemus tetraspis) and the slender-snouted crocodile (Mecistops cataphractus) was assessed using a sheep red blood cell (SRBC) hemolytic assay. The hemolysis for both crocodilian species was serum volume-, time-, and temperature-dependent. Although the serum volume-dependent activities were similar for both species (CH50 = 81 μL for Osteolaemus tetraspis and 96 μL for Mecistops cataphractus), the kinetic curves show a greater amount cooperativity, and thus more rapid SRBC lysis, for Osteolaemus tetraspis. In addition, the hemolytic activities were very similar at 10 – 35°C, but the serum from Osteolaemus tetraspis was more active than that of Mecistops cataphractus at the temperature extremes tested (5°C and 40°C). The activities for both species were almost completely inhibited by 1 mM EDTA, indicating the dependency on divalent metal ions. However, the EDTA-inhibited hemolysis could be restored by the addition of excess Mg2+ and Ca2+, but not Ba2+, Cu2+, or Fe2+, which exhibited the specificity for Mg2+or Ca2+. These data indicate that these sympatric, but evolutionarily and ecologically divergent crocodile species have similar SRBC hemolysis activities with similar mechanisms, thus reinforcing the idea that serum complement is an ancient, innate immunity host defense system.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

https://www.gaziantepescort.info

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7