alexa Short-Term Impact of Starch Particles on Endometriotic Cells in vitro and in a Xenograft Nude Mouse Model | OMICS International | Abstract
ISSN: 2161-0932

Gynecology & Obstetrics
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Research Article

Short-Term Impact of Starch Particles on Endometriotic Cells in vitro and in a Xenograft Nude Mouse Model

Sjosten ACE1, Gogusev J2, Malm E3, Sonden A1, Ingelman-Sundberg H4, Kjellstrom BT3 and Edelstam GAB5*

1Department of Clinical Science and Education, Sodersjukhuset, Karolinska Institutet, Stockholm, Sweden

2INSERM U507, Hopital Necker, Paris, France

3Department of Defense Medicine, Defense Research Agency, Stockholm, Sweden

4Department of Pathology at Danderyd University Hospital, Stockholm, Sweden

5Department of Obstetrics & Gynecology, Uppsala University Hospital, Sweden

Corresponding Author:
Edelstam GAB
Department of Obstetrics & Gynecology
Uppsala University Hospital, Sweden
E-mail: [email protected]

Received Date: May 25, 2013; Accepted Date: July 05, 2013; Published Date: July 08, 2013

Citation: Sjosten ACE, Gogusev J, Malm E, Sonden A, Ingelman-Sundberg H, et al. (2013) Short-Term Impact of Starch Particles on Endometriotic Cells in vitro and in a Xenograft Nude Mouse Model. Gynecol Obstet 3:154. doi: 10.4172/2161-0932.1000154

Copyright: © 2013 Sjosten ACE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Purpose: Investigating the impact of starch powder on human endometriotic cells in vitro and on the implantation and development of adhesions in vivo.
Basic procedure: In vitro - A human endometriotic cell line was incubated with different concentrations of starch particles and the effect on growth was measured by immunoassay. In vivo - Cells from a human endometriotic cell culture were injected with and without starch, into the peritoneal cavity of nude mice investigated 8, 15 respectively 22 days later.
Main findings: In vitro - A low dose of starch particles significantly inhibited the proliferation of endometriotic cells during incubation for 48 h. In vivo - There were significant differences in the development of adhesions when the endometriotic cells were injected intraperitoneally together with starch particles. The development of endometriotic implants was delayed but not significantly in the presence of starch particles.
Principal conclusions: Starch particles have a negative impact on the proliferation of endometriotic cells in vitro and affect the development of endometriotic implants in vivo.


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