Sibling Oocytes Randomly Assigned to Either Conventional Fertilization or Intracytoplasmic Sperm Injection Demonstrate Equivalent Fertilization and Blastulation Rates
|Kyle J Tobler1, Paul Brezina2*, Jairo E Garcia1 and Yulian Zhao1|
|1 Johns Hopkins University School of Medicine, Department of Gynecology and Obstetrics, Division of Reproductive Endocrinology and Infertility, Baltimore, USA|
|2 Assistant Clinical Professor of Reproductive Endocrinology and Infertility, Vanderbilt University School of Medicine, USA|
|*Corresponding Author :||Paul Brezina, MD/MBA
Assistant Clinical Professor of Reproductive Endocrinology and Infertility
Vanderbilt University School of Medicine, USA
Email: [email protected]
|Received March 03, 2014; Accepted May 27, 2014; Published May 29, 2014|
|Citation: Tobler KJ, Brezina P, Garcia JE, Zhao Y (2014) Sibling Oocytes Randomly Assigned to Either Conventional Fertilization or Intracytoplasmic Sperm Injection Demonstrate Equivalent Fertilization and Blastulation Rates. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 2:124. doi:10.4172/jfiv.1000124|
|Copyright: © 2014 Tobler KJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Background: To determine the difference in fertilization and blastulation rates between sibling oocytes randomly assigned to either Conventional Fertilization (CF) or Intracytoplasmic Sperm Injection (ICSI) in patient couples with normal semen analysis parameters.
Methods: A retrospective review of embryologic and clinical outcomes from patients undergoing their first In Vitro Fertilization (IVF) cycle that randomly assigned one-half of sibling oocytes to either CF or ICSI. Randomization occurred prior to removal of the oocyte cumulus cells which allows identification of metaphase II oocytes eligible for ICSI. All male partners met normal semen analysis parameters and normal fertilization was anticipated for the IVF cycle. Each patient served as their own control.
Results: A total of 682 oocytes were included and were assigned to either CF or ICSI. The CF had a significantly higher fertilization rate compared to ICSI (67.9% versus 60%) when immature (non-metaphase II) oocytes were included in the analysis; however this difference diminished when only those oocytes eligible for ICSI were included. There was no difference in the day-3 embryo progression, the blastulation rate, the mean number of oocytes fertilized and the mean number of blastocysts per IVF cycle. Additionally, there was no significant difference in the pregnancy related outcomes between the two groups.
Conclusions: These studies demonstrates that in patient couples with normal semen analysis parameters, undergoing their first IVF cycle and who have anticipate normal fertilization, randomly assigning oocytes to CF or ICSI does not improve fertilization or blastulation. To confirm these findings, larger prospective studies are required.