Signaling Networks in Gastric Cancer Cells Revealed by Phosphoproteomics
Guang-Rong Yan, Nan-Peng Chen, Ya-Dong Huang, Wen Ding, Gui-Wei He, Chuan-Le Xiao, Xing-Feng Yin, Qing-Yu He*
Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, Guangzhou 510632, China
- *Corresponding Author:
- Prof. Qing-Yu He
Institute of Life and Health Engineering
and National Engineering and Research Center for
Genetic Medicine Jinan University
Guangzhou 510632, China
E-mail: [email protected]
Received Date: January 20, 2010; Accepted Date: April 08, 2010; Published Date: April 08, 2010
Citation: Yan GR, Chen NP, Huang YD, Wen D, He GW, et al. (2010) Signaling Networks in Gastric Cancer Cells Revealed by Phosphoproteomics. J Proteomics Bioinform 3: 113-120. doi: 10.4172/jpb.1000129
Copyright: © 2010 Yan GR, et al. This is an open-access Article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Signaling molecules in signaling pathways proceed to be phosphorylated in the signal transmission from membrane receptors to nuclus. Dysregulated phosphorylation has been implicated in a variety of human diseases including cancer. In this study, we combined SDS-PAGE prefractionation, TiO2 phosphopeptide enrichment and LC-MS/MS technologies to identify the phosphoproteins and their regulatory sites in human gastric cancer cells. Totally 282 phosphorylation sites, corresponding to 245 unique peptides and 161 different proteins, were identified after the evaluation of ambiguous phosphosites. Among them, the phosphorylation of 109 (38.7%) sites and 36 (22.4%) proteins has not been previously reported. It was found that EGFR-ERK1/2 signaling network was mainly involved in the phosphorylation regulation of the identified proteins, suggestting that EGFR-ERK1/2 pathway plays a critical role in the network controlling gastric cancer cell process, and thus may be a drug target of anti-gastric cancer.