Significant Association of Proline Allele in Tp53 Gene, Arg72pro Polymorphism in Brain Tumors and its Prognostic Value
|Aamir Allam Khan1#, Arshad A Pandith2#, Abrar Ahad Wani1, Nayil Khursheed Malik1, Zafar Amin Shah3, Mosin S Khan5, Farhanaz Mehraj1, Yousuf Kachroo1, Arif H Sarmast1, and Altaf Umar Ramzan1*|
|1Department of Neurosurgery, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India|
|2Advanced Centre for Human Genetics, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India|
|3Department of Immunology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India|
|4Department of General Surgery, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India|
|5Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India|
|Corresponding Author :||Altaf Umar Ramzan
Department of Neurosurgery, Sher-I-Kashmir Institute of Medical Sciences
Soura, Srinagar, J and K, Pin Code 190011, India
E-mail: [email protected], [email protected]
|Received: May 28, 2015; Accepted: July 17, 2015; Published: July 21, 2015|
|Citation: Khan AA, Pandith AA, Wani AA, Malik NK, Shah ZA (2015) Significant Association of Proline Allele in Tp53 Gene, Arg72pro Polymorphism in Brain Tumors and its Prognostic Value. J Cancer Sci Ther 7: 236-241. doi:10.4172/1948- 5956.1000355|
|Copyright: © 2015 Khan AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: In TP53 gene, Arg72Pro polymorphism has been suggested to be associated with genetically determined susceptibility in various types of cancers including brain tumors. Our objective was to investigate the possible association between TP53 Arg72Pro polymorphism with brain tumor susceptibility and to examine its correlation with the clinico-pathologic variables of tumor cases for oncologic prognosis of patients.
Materials and methods: The TP53 Arg72Pro genotypes were determined by Polymerase Chain Reaction- Based Restriction Fragment Length Polymorphism (PCR-RFLP) analysis in 90 age and gender matched brain tumor cases and 130 healthy controls.
Results: We found significant difference in the frequency of Pro allele as 0.47 in cases versus 0.37 in controls (p<0.05).Though the distribution of the TP53, 72Pro genotypes in patients were higher (24.4%) compared with the control group (17.7%) with an odds ratio of 1.9 but no statistical difference was found (p>0.05). Proline related allele/genotype (GC) was significantly found associated in high grade malignant brain tumors, neuroepithelial tumors (p<0.05). Overall and disease-free survivals were calculated but no significant statistical difference was observed between the two groups (p>0.05).
Conclusion: Though overall distribution did not reveal significant association in TP53 Arg72Pro polymorphism among cases and controls but proline related genotype was found associated with susceptibility to the disease pathology.