alexa Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay

Felipe Rebello Lourenço*

Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil

*Corresponding Author:
Felipe Rebello Lourenço
Departamento de Farmácia
Faculdade de Ciências Farmacêuticas
Universidade de São Paulo, Brazil
E-mail: [email protected]

Received date: November 09, 2012; Accepted date: November 27, 2012; Published date: November 30, 2012

Citation: Lourenço FR (2012) Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay. J Chromat Separation Techniq 3:153. doi:10.4172/2157-7064.1000153

Copyright: © 2012 Lourenço FR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

This work proposes a procedure to estimate the measurement uncertainty for the quantification of cefazolin by high-performance liquid chromatography (HPLC) and microbiological assay (bioassay). HPLC method was performed according to United States Pharmacopeia (USP) and bioassay according to Brazilian Pharmacopeia (FB). The quantification of cefazolin by high-performance liquid chromatography was assess through the calibration curve equation (Y=0.0199 X–0.0323). The HPLC uncertainty was estimated based on the error of the slope and the intercept (between 4.4% and 5.3%). The bioassay uncertainty was estimated based on the standard deviation of inhibition zones of samples and standards solutions (between 6.2 and 6.7%). The uncertainties for bioassay are expected to be higher those obtained by HPLC. Both HPLC method and bioassay estimated uncertainties are reasonable for the scope of each method.

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