alexa Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay

Felipe Rebello Lourenço*

Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil

*Corresponding Author:
Felipe Rebello Lourenço
Departamento de Farmácia
Faculdade de Ciências Farmacêuticas
Universidade de São Paulo, Brazil
E-mail: [email protected]

Received date: November 09, 2012; Accepted date: November 27, 2012; Published date: November 30, 2012

Citation: Lourenço FR (2012) Simple Estimation of Uncertainty in the Quantification of Cefazolin by HPLC and Bioassay. J Chromat Separation Techniq 3:153. doi:10.4172/2157-7064.1000153

Copyright: © 2012 Lourenço FR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



This work proposes a procedure to estimate the measurement uncertainty for the quantification of cefazolin by high-performance liquid chromatography (HPLC) and microbiological assay (bioassay). HPLC method was performed according to United States Pharmacopeia (USP) and bioassay according to Brazilian Pharmacopeia (FB). The quantification of cefazolin by high-performance liquid chromatography was assess through the calibration curve equation (Y=0.0199 X–0.0323). The HPLC uncertainty was estimated based on the error of the slope and the intercept (between 4.4% and 5.3%). The bioassay uncertainty was estimated based on the standard deviation of inhibition zones of samples and standards solutions (between 6.2 and 6.7%). The uncertainties for bioassay are expected to be higher those obtained by HPLC. Both HPLC method and bioassay estimated uncertainties are reasonable for the scope of each method.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version