Simultaneous Determination of Carvedilol and Hydrochlorothiazide in Tablets and Spiked Human Plasma using Derivative SpectrophotometryAida Ansary1, Mona M. Abdel-Moety2*, Fatma M. Abdel-Gawad2, Ether A. Mohamed2 and Motaza M. Khater1
- *Corresponding Author:
- Mona M. Abdel-Moety
National Organization for Drug Control and Research
P.O. Box 29, Analytical Chemistry Department
E-mail: [email protected]
Received date: November 09, 2012; Accepted date: November 21, 2012; Published date: November 25, 2012
Citation: Ansary A, Abdel-Moety MM, Abdel-Gawad FM, Mohamed EA, Khater MM (2012) Simultaneous Determination of Carvedilol and Hydrochlorothiazide in Tablets and Spiked Human Plasma using Derivative Spectrophotometry. Pharmaceut Anal Acta 3:186. doi: 10.4172/2153-2435.1000186
Copyright: © 2012 Ansary A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Quick and accurate methods for determination of carvedilol and hydrochlorothiazide in tablets and spiked human plasma by using third derivative (3D), fourth derivative (4D) and ratio spectra derivative (1DD) spectrophotometric methods were developed. The zero-crossing technique was employed in measurements, using 3D at 245.9 or 230.2 nm and 4D at 247.4 or 226.9 nm for carvedilol or hydrochlorothiazide, respectively. The first-derivative of ratio spectra (1DD) where the amplitudes were measured at 236.1 nm for carvedilol and 261.1 nm for hydrochlorothiazide. The calibration curves were linear in the ranges of 1.0-20.0 μg mL-1 for each of carvedilol and hydrochlorothiazide using 3D 4D and 1DD methods. The suggested methods were tested using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulations. The methods retained their accuracy and precision when the standard addition technique was applied. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by reported and official HPLC methods for carvedilol and hydrochlorothiazide, respectively. These methods showed to be appropriate for simultaneous determination of carvedilol and hydrochlorothiazide in human plasma samples with a limit of quantitation (LOQ) is ≤ 0.5 μg mL-1.