Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid ChromatographyLakshmana Prabu S*, M. Srinivasan, S Thiagarajan and Queeni Marina
Department of Pharmaceutical Technology, Anna University Tiruchirappalli, Tiruchirappalli 620024 India
- *Corresponding Author:
- Dr. Lakshmana Prabu S
Department of Pharmaceutical Technology
Anna University Tiruchirappalli
Tiruchirappalli 620024, India
E-mail: [email protected]
Received Date: August 30, 2010; Accepted Date: September 27, 2010; Published Date: September 30, 2010
Citation:Prabu SL, Srinivasan M, Thiagarajan S, Marina Q (2010) Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid Chromatography. Pharm Anal Acta 1:110.doi: 10.4172/2153-2435.1000110
Copyright: © 2010 Prabu SL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..
A rapid and accurate liquid chromatographic method has been developed for the simultaneous determination of gatifloxacin (GFC) and ambroxol hydrochloride (AMB) in a tablet formulation. Chromatographic separation of the two drugs was achieved on a Phenomenex column (200mm×4.6 mm, 5μm). The mobile phase consisting of a mixture of 0.1 M phosphate buffer adjusted to pH 5.5 and acetonitrile in the ratio of 55:45 was delivered at a flow rate of 1.0 ml/ min. Detection was performed at 254 nm using UV detector. The retention time for GFC was around 2.2 and AMB was around 4.5 min; separation was complete in less than 10 min. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range of 10 – 200 μg/ml and 10 – 100 μg/ml and correlation coefficient was found to be 0.9992 and 0.9983 for GFC and AMB respectively. The method was validated for accuracy, precision and recovery studies. Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GFC and AMB. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quality control of formulation products.