alexa Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid Chromatography
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid Chromatography

Lakshmana Prabu S*, M. Srinivasan, S Thiagarajan and Queeni Marina

Department of Pharmaceutical Technology, Anna University Tiruchirappalli, Tiruchirappalli 620024 India

*Corresponding Author:
Dr. Lakshmana Prabu S
Department of Pharmaceutical Technology
Anna University Tiruchirappalli
Tiruchirappalli 620024, India
E-mail: [email protected]

Received Date: August 30, 2010; Accepted Date: September 27, 2010; Published Date: September 30, 2010

Citation:Prabu SL, Srinivasan M, Thiagarajan S, Marina Q (2010) Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid Chromatography. Pharm Anal Acta 1:110.doi: 10.4172/2153-2435.1000110

Copyright: © 2010 Prabu SL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..

Abstract

A rapid and accurate liquid chromatographic method has been developed for the simultaneous determination of gatifloxacin (GFC) and ambroxol hydrochloride (AMB) in a tablet formulation. Chromatographic separation of the two drugs was achieved on a Phenomenex column (200mm×4.6 mm, 5μm). The mobile phase consisting of a mixture of 0.1 M phosphate buffer adjusted to pH 5.5 and acetonitrile in the ratio of 55:45 was delivered at a flow rate of 1.0 ml/ min. Detection was performed at 254 nm using UV detector. The retention time for GFC was around 2.2 and AMB was around 4.5 min; separation was complete in less than 10 min. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range of 10 – 200 μg/ml and 10 – 100 μg/ml and correlation coefficient was found to be 0.9992 and 0.9983 for GFC and AMB respectively. The method was validated for accuracy, precision and recovery studies. Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GFC and AMB. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quality control of formulation products.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords