alexa Simultaneous Determination of Seven Bioactive Compounds in Wuji Pill by HPLC | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Simultaneous Determination of Seven Bioactive Compounds in Wuji Pill by HPLC

Yuan Gao1, Feng-Yun Jin2*, Xiang-Pei Wang1, Yang Zhao2 and Guang-Yi Liang1

1Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China

2West China School of Pharmacy, Sichuan University, Chengdu 610041, China

*Corresponding Author:
Feng-Yun Jin
Guiyang College of Traditional Chinese Medicine
Guiyang 550001, China
Tel: +86 851 5615344
Fax: +86 851 5615344
E-mail: [email protected]

Received date: May 15, 2012; Accepted date: August 16, 2012; Published date: August 18, 2012

Citation: Gao Y, Jin FY, Wang XP, Zhao Y, Liang GY (2012) Simultaneous Determination of Seven Bioactive Compounds in Wuji Pill by HPLC. J Chromat Separation Techniq 3:132. doi:10.4172/2157-7064.1000132

Copyright: © 2012 Gao Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A high performance liquid chromatography coupled with photodiode-array detection method was developed for simultaneous determination of gallic acid, chlorogenic acid, paeoniflorin, jatrorrhizine hydrochloride, dehydroevodiamine, palmatine chloride and berberine hydrochloride in the Chinese proprietary medicine “Wuji Pill” (WJP). The analysis was performed by reversed phase gradient elution, using an aqueous mobile phase (containing 0.05 mol/L monopotassium phosphate and 0.1% aqueous phosphosnic acid) modified by acetonitrile and detection made at 340 nm. The method was validated for limits of detection and quantification, precision, repeatability, stability, accuracy and robustness. Five batches of self-manufactured WJP were analyzed and found to contain different amounts of the seven bioactive markers. The method could be used for quality assessment of this Chinese materia medica preparation.

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