Simultaneous Determination of Seven Bioactive Compounds in Wuji Pill by HPLC
- *Corresponding Author:
- Feng-Yun Jin
Guiyang College of Traditional Chinese Medicine
Guiyang 550001, China
Tel: +86 851 5615344
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E-mail: [email protected]
Received date: May 15, 2012; Accepted date: August 16, 2012; Published date: August 18, 2012
Citation: Gao Y, Jin FY, Wang XP, Zhao Y, Liang GY (2012) Simultaneous Determination of Seven Bioactive Compounds in Wuji Pill by HPLC. J Chromat Separation Techniq 3:132. doi:10.4172/2157-7064.1000132
Copyright: © 2012 Gao Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A high performance liquid chromatography coupled with photodiode-array detection method was developed for simultaneous determination of gallic acid, chlorogenic acid, paeoniflorin, jatrorrhizine hydrochloride, dehydroevodiamine, palmatine chloride and berberine hydrochloride in the Chinese proprietary medicine “Wuji Pill” (WJP). The analysis was performed by reversed phase gradient elution, using an aqueous mobile phase (containing 0.05 mol/L monopotassium phosphate and 0.1% aqueous phosphosnic acid) modified by acetonitrile and detection made at 340 nm. The method was validated for limits of detection and quantification, precision, repeatability, stability, accuracy and robustness. Five batches of self-manufactured WJP were analyzed and found to contain different amounts of the seven bioactive markers. The method could be used for quality assessment of this Chinese materia medica preparation.