Simultaneous Determination of Six Insecticides in Okra (Abelmoschus esculentus L. Moench) by UHPLC-MS/MS
- *Corresponding Author:
- Yongquan Zheng
Institute of Plant Protection, Chinese Academy of Agricultural Sciences
Key Laboratory of Pesticide Chemistry and Application
Ministry of Agriculture; Beijing 100193, P.R China
Tel: +86-01- 62815938
E-mail: [email protected]
Received Date: June 14, 2017; Accepted Date: June 21, 2017; Published Date: June 27, 2017
Citation: Hussan HNM, Liu X, Dong F, Xu J, Zheng Y (2017) Simultaneous Determination of Six Insecticides in Okra (Abelmoschus esculentus L. Moench) by UHPLC-MS/MS. J Chromatogr Sep Tech 8:370. doi: 10.4172/2157-7064.1000370
Copyright: © 2017 Hussan HNM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The sensitive analytical method using quick, easy, cheap, effective, rugged and safe (QuEChERS) method for simultaneous determination of six insecticides in Okra was developed by UHPLC -MS-MS. The six insecticides were extracted from Okra matrices using acetonitrile and subsequently clean up using only octadecylsilance as sorbent prior to LC-MS-MS analysis. The determination of the target compounds were achieved in less than 2.0 min using an electrospray ionization source in positive mode (ESI+) for imidacloprid, acetamiprid, thiacloprid, thiamethoxam, clothianidin and negative mode (ESI-) for flonicamid. The method showed that the limits quantification (LOQ) ranged from 0.13 to 5.9 μgkg-1 in all matrices. The matrix-matched standard gave satisfactory recoveries (72.4%-105.1%) and relative standard deviation (2.2 - 20.0%) values in different matrices at three spiked levels (0.01, 0.1, and 1 mgkg-1) for Okra.