Simultaneous Estimation of Ambroxol Hydrochloride and Doxofylline in Pharmaceutical Formulation by HPTLC-Desitometric Method
Falgun A Mehta, Emanual Michael Patelia* and Pradip N Bhoya
Department of Pharmaceutical Chemistry and Analysis, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar–388121, Gujarat, India
- *Corresponding Author:
- Emanual Michael Patelia
Department of Pharmaceutical Chemistry and Analysis
Indukaka Ipcowala College of Pharmacy
New Vallabh Vidyanagar–388121
E-mail: [email protected]
Received Date: January 11, 2013; Accepted Date: January 29, 2013; Published Date: January 31, 2013
Citation: Mehta FA, Patelia EM, Bhoya PN (2013) Simultaneous Estimation of Ambroxol Hydrochloride and Doxofylline in Pharmaceutical Formulation by HPTLCDesitometric Method. J Chromat Separation Techniq 4:168. doi:10.4172/2157-7064.1000168
Copyright: © 2013 Mehta FA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Ambroxol Hydrochloride and Doxofylline. The HPTLC method was performed on precoated silica gel G60 F254 plates with Diethyl ether:n-butanol:Ammonia (9:0.9:0.1 v/v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their absorption at approximately 254 nm for AMB and DOX. The drugs were satisfactorily resolved with Rf 0.29 ± 0.02 for and Rf 0.56 ± 0.02 for DOX. The calibration plot was linear between 100- 600 ng /band for AMB and 200-1200 ng/band based for DOX. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined dosage form.