alexa Simultaneous Estimation of Pregabalin and Methylcobalamine in Pharmaceutical Formulation by HPTLC-Densitometry Method
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Simultaneous Estimation of Pregabalin and Methylcobalamine in Pharmaceutical Formulation by HPTLC-Densitometry Method

Dimal A Shah, Emanual Michael Patelia* and Aswin Mori

Department of Pharmaceutical Chemistry and Analysis, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar–388121, Gujarat, India

*Corresponding Author:
Emanual Michael Patelia
Department of Pharmaceutical Chemistry and Analysis
Indukaka Ipcowala College of Pharmacy
New Vallabh Vidyanagar–388121
Gujarat, India
E-mail: [email protected]

Received Date: January 11, 2013; Accepted Date: January 29, 2013; Published Date: January 31, 2013

Citation: Shah DA, Patelia EM, Mori A (2013) Simultaneous Estimation of Pregabalin and Methylcobalamine in Pharmaceutical Formulation by HPTLCDensitometry Method. J Chromat Separation Techniq 4:169. doi:10.4172/2157-7064.1000169

Copyright: © 2013 Shah DA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of PRG and MC. HPTLC method was developed using on precoated silica gel F254 G60 plates as stationary phase, using methanol:toluene:ammonia (30%) (8:2:0.4 v/v/v) as mobile phase. The plates were scanned at approximately 497 nm for both PRG and MC respectively. In HPTLC method both the drugs were resolved using proposed mobile phase and Rf value was found to be 0.60 for PRG and Rf 0.33 for MC. The method was found to linear in the range 1500-7500 ng/band for PRG, 150-750 ng/ band based for MC respectively. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is important tool for analysis of combined dosage form.


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