Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream FormulationsAdnan Manassra1*, Mustafa Khamis1, Magdy el-Dakiky1, Zuhair Abdel-Qader2 and Fuad Al-Rimawi1
- *Corresponding Author:
- Dr. Adnan Manassra
Faculty of Science and Technology
Al-Quds University, P.O. Box 20002
E-mail: [email protected]
Received Date: August 30, 2010; Accepted Date: October 11, 2010; Published Date: December 30, 2010
Citation: Manassra A, Khamis M, el-Dakiky M, Abdel-Qader Z, Al-Rimawi F (2010) Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations. Pharm Anal Acta 1:113 doi: 10.4172/2153-2435.1000113
Copyright: © 2010 Manassra A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
An HPLC method for the simultaneous quantitative determination of betamethasone and clotrimazole in cream formulation has been developed. The method utilizes a reversed-phase C18 (250 X 4.0 mm) stationary phase, with a mixture of methanol-acetate buffer-acetonitrile (33:27:40, v/v) as a mobile phase, and spectrophotometric UV detection at 254 nm. The method has been validated for cream formulations containing betamethasone and clotrimazole with linear range of 0.025 to 0.075 mg/ml for betamethasone with a correlation coefficient of 0.9996, and linear range of 0.25 to 0.75 mg/ml for clotrimazole with a correlation coefficient of 1.000. The results demonstrated that this method is accurate, precise, specific, linear, reliable, sensitive, and fast.