Simultaneous Liquid Chromatographic Determination of Two Co- Prescribed Anti-Cancer Drugs in Bulk Drug, Dosage Formulations and in Human Serum Using Multivariate Technique: Application to in vitro Drug InteractionNajma Sultana1, Saeed Arayne M2, Saeeda Nadir Ali2* and Arman Tabassum2
- *Corresponding Author:
- Saeeda Nadir Ali
Department of Chemistry
University of Karachi, Karachi-75270
E-mail: [email protected]
Received date: January 30, 2013; Accepted date: March 20, 2013; Published date: March 25, 2013
Citation: Sultana N, Saeed Arayne M, Nadir Ali S, Tabassum A (2013) Simultaneous Liquid Chromatographic Determination of Two Co-Prescribed Anti- Cancer Drugs in Bulk Drug, Dosage Formulations and in Human Serum Using Multivariate Technique: Application to in vitro Drug Interaction. Pharmaceut Anal Acta 4:215. doi: 10.4172/2153-2435.1000215
Copyright: © 2013 Sultana N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Present study describes the development and validation of a high-performance liquid chromatographic (HPLC) assay for the simultaneous quantification of atorvastatin and celecoxib in human serum and pharmaceutical formulations. The separation was carried out on Bondapak, C18 (10 μm, 25×0.46 cm) column using mobile phase 80:20 methanol-water of pH 3.5, maintained with o-phosphoric acid. The method was optimized using multivariate technique and detector response was recorded at five different wavelengths. Chromatography was performed at ambient temperature by pumping the mobile phase at a flow rate of 1.0 mL min-1. Calibration curves were found to be linear with correlation coefficient greater than 0.998 over the concentration range 2-60 and 2-70 ng mL-1 with detection limits 0.024 and 0.019 ng mL-1 in bulk drug and 0.04 and 0.01 ng mL-1 in serum at isobestic point. The method was validated for specificity, selectivity, and linearity, accuracy, precision and intermediate precision. The proposed method is valid for the determination of drugs in pharmaceutical formulations without interference of excipients. Recovery values of both drugs were 99.33 to 101.65%. Moreover the in vitro interaction studies of atorvastatin in presence of celecoxib were carried out at physiological temperature (37°C), simulating stomach environment and the reactions were studied by RP-HPLC.