Simultaneous Production of Xylitol and Ethanol from Different Hemicellulose Waste Substrates by Candida tropicalis Strain Ly15
Kaliyan Barathikannan, Ameer Khusro and Agastian Paul*
Research Department of Plant Biology and Biotechnology, School of Life Science, Loyola College, Nungambakkam, Chennai, Tamil Nadu, India
- Corresponding Author:
- Agastian Paul
Research Department of Plant Biology
and Biotechnology, School of Life Science, Loyola College
Nungambakkam, Chennai-34, Tamil Nadu, India
E-mail: [email protected]
Received date: October 31, 2016; Accepted date: November 28, 2016; Published date: November 30, 2016
Citation: Barathikannan K, Khusro A, Paul A (2016) Simultaneous Production of Xylitol and Ethanol from Different Hemicellulose Waste Substrates by Candida tropicalis Strain Ly15. J Bioprocess Biotech 6:289. doi:10.4172/2155-9821.1000289
Copyright: © 2016 Barathikannan K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In the present study, we optimized the parameters for maximum yield of xylitol and ethanol by Candida tropicalis strain LY15 utilizing different Hemicellulosic Waste substrates (HWs) obtained by Acid-Alkaline hydrolysis method. Using the pomegranate peel as substrate, higher yield of xylose reductase, xylitol and ethanol were obtained. The optimal level of variables such as inoculum, HWs concentration, time period and RPM (agitation speed) were determined by the response surface methodology, with a significant co-efficient determination of R2= 0.89. Positive correlation was obtained with our experimental and predicted values. The interactions with the ideal levels of inoculum (1%), HWs (Pomegranate peel) (2%), time period (60 h) and RPM (140) showed highly significance values of xylose reductase (171.5 IU/mL), xylitol (55.57 g l-1) and ethanol (3.37 g l-1) at an incubation period of 60 h. The dual yield produced by Candida tropicalis strain LY15 using pomegranate peel makes the organism potent candidate for industrial application.