Simultaneous Quantitative Determination of Nine Bufadienolides in Traditional Chinese Medicinal Toad Skin from Different Regions of China by High-Performance Liquid ChromatographyÂ–Photodiode Array DetectionSi-Wang Wang*, Lin-Rui Duan, Wei Cao, Yan-Hua Xie, Jia-Ni Yuan, Hua Li and Xiao-Kai Zhang
Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, Xi’an 710032, Shaanxi, China
- *Corresponding Author:
- Si-wang Wang
Department of Natural Medicine, School of Pharmacy
Fourth Military Medical University
169 West Changle Road, Xi’an 710032, Shaanxi, China
Tel: +86 29 8322 4790
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E-mail: [email protected]
Received date: December 29, 2014; Accepted date: March 03, 2015; Published date: March 10, 2015
Citation: Wang SW, Duan LR, Cao W, Xie YH, Yuan JN, et al. (2015) Simultaneous Quantitative Determination of Nine Bufadienolides in Traditional Chinese Medicinal Toad Skin from Different Regions of China by High-Performance Liquid Chromatography–Photodiode Array Detection.Pharm Anal Acta 6:345. doi: 10.4172/2153-2435.1000345
Copyright: © 2015 Wang SW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Toad skin is a traditional Chinese medicine for the treatment of various tumors. The major active components in toad skin are bufadienolides. In this paper, a simple, accurate and reliable method for the simultaneous separation and determination of nine active components (gamabufotalin, arenobufagin, telocinobufagin, desacetylcinobufotalin, bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogeni) in toad skin was developed using High-Performance Liquid Chromatography (HPLC) coupled with Photodiode Array Detection (PDA) detection. The chromatographic separation was performed on a SinoChrom ODS-BP C18 column with gradient elution using acetonitrile and 0.1% acetic acid-0.5% potassium dihydrogen phosphate aqueous solution at a flow rate of 0.8 mL min-1. All compounds showed good linearity in a wide concentration range with the values of r2 higher than 0.9994, and their limits of detection were at the range of 0.06-0.10 μg mL-1. The separation and identification of the nine bufadienolides in this paper may provide important experimental data for further research and application of toad skin.