alexa Single Cell Protein Production Using Penicillium ochroc
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Single Cell Protein Production Using Penicillium ochrochloron Chitinase and Its Evaluation in Fish Meal Formulations

Nilambari S Patil and Jyoti P Jadhav*

Department of Biotechnology, Shivaji University, Kolhapur, India

*Corresponding Author:
Prof. Mrs. Jyoti P. Jadhav
Professor and Head
Department of Biotechnology
Shivaji University, Vidyanagar
Kolhapur 416004, India
Tel: +912312609365
Fax: +912311691533
E-mail: [email protected]

Published date: July 19, 2014; Published date: September 04, 2014; Published date: September 11, 2014

Citation: Patil NS, Jadhav JP (2014) Single Cell Protein Production Using Penicillium ochrochloron Chitinase and Its Evaluation in Fish Meal Formulations. J Microb Biochem Technol S4:005. doi:10.4172/1948-5948.S4-005

Copyright: © 2014 Patil NS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

The conversion of the enzymatic hydrolysate of shellfish chitin to single-cell protein was investigated. The endproduct of chitin hydrolysis by Penicillium ochrochloron chitinase was mainly N-acetyl-D-glucosamine, its further utilization as a substrate for SCP production using Yerrowia lipolytica NCIM 3450 has been studied. The 2% chitin hydrolysate was found to be optimal for SCP production. A biomass of 9.4 g/l, total protein and nucleic acid content of the biomass was 65% and 2.9%, respectively. Fish diets were formulated to replace fishmeal partially by single cell protein from Yerrowia lipolytica using chitin hydrolysate in diets of Lepidocephalus thermalis for a period of 2 weeks. Control diet and three experimental diets were prepared to replace 25%, 50% and 75% of fish meal using SCP. The result indicated 50% yeast SCP diet gave the better growth response in Lepidocephalus thermalis than other formulations.

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