Single Cell Protein Production Using Penicillium ochrochloron Chitinase and Its Evaluation in Fish Meal Formulations*Corresponding Author: Prof. Jyoti P Jadhav, Professor and Head, Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004, India, Tel: +912312609365, Fax: +912311691533, Email: [email protected]
Received Date: Jul 19, 2014 / Accepted Date: Sep 04, 2014 / Published Date: Sep 11, 2014
Citation: Patil NS, Jadhav JP (2014) Single Cell Protein Production Using Penicillium ochrochloron Chitinase and Its Evaluation in Fish Meal Formulations. J Microb Biochem Technol S4:005.DOI: 10.4172/1948-5948.S4-005
Copyright: © 2014 Patil NS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
The conversion of the enzymatic hydrolysate of shellfish chitin to single-cell protein was investigated. The endproduct of chitin hydrolysis by Penicillium ochrochloron chitinase was mainly N-acetyl-D-glucosamine, its further utilization as a substrate for SCP production using Yerrowia lipolytica NCIM 3450 has been studied. The 2% chitin hydrolysate was found to be optimal for SCP production. A biomass of 9.4 g/l, total protein and nucleic acid content of the biomass was 65% and 2.9%, respectively. Fish diets were formulated to replace fishmeal partially by single cell protein from Yerrowia lipolytica using chitin hydrolysate in diets of Lepidocephalus thermalis for a period of 2 weeks. Control diet and three experimental diets were prepared to replace 25%, 50% and 75% of fish meal using SCP. The result indicated 50% yeast SCP diet gave the better growth response in Lepidocephalus thermalis than other formulations.