Single Nucleotide Polymorphism of IL4C-590T and IL4RA 175V and Immunological Parameters in Egyptian Asthmatics with Different Clinical Phenotypes
Magdy Zedan*, Ashraf Bakr, Basma Shouman, Hosam Zaghloul, Mohammad Al-Haggar, Mohamed Zedan and Amal Osman
Mansoura Faculty of Medicine, Mansoura, Egypt
- *Corresponding Author:
- Magdy Zedan
Mansoura Faculty of Medicine
Post Office: 35516
Box 50, Mansoura, Egypt
Tel: 20502242526, 201223226535
E-mail: [email protected]
Received date: July 05, 2014; Accepted date: August 12, 2014; Published date: August 19, 2014
Citation: Zedan M, Bakr A, Shouman B, Zaghloul H, Al-Haggar M, et al. (2014) Single Nucleotide Polymorphism of IL4C-590T and IL4RA 175V and Immunological Parameters in Egyptian Asthmatics with Different Clinical Phenotypes. J Allergy Ther 5:189. doi: 10.4172/2155-6121.1000189
Copyright: 2014 Zedan M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objectives: Circulating cytokines and genetic patterns may help to discriminate certain asthma phenotypes.
Methods: Eighty two uncontrolled asthmatic children and twenty controls were enrolled in the study. After validation of asthma symptoms, three proposed phenotypes were formed: cough, Shortness Of Breath (SOB), and cough with SOB. Measurement of pulmonary function tests, Fractional Exhaled Nitric Oxide (FENO), eosinophilic percentage, serum levels of total IgE, IL-17 and IL-9 were done. Two Single-Nucleotide Polymorphisms (SNPs) in IL4 and IL4RA were genotyped using PCR-RFLP method.
Results: Regarding SNP IL4RA-175V, cases showed heterozygous AG predominance whereas controls showed more homozygous GG genotype. Cough group showed significant decrease in both FEV1/FVC ratio in comparison to SOB and cough with SOB groups. Also, this group showed strong inverse relationship between FEV1 values and serum IL-9. In addition, there was significant increase in serum levels of IL-17 among homozygous CC compared to CT heterozygous patients of SNP IL-4C 590T in both cough group and SOB group. Cough with SOB group showed significant increase of serum levels of IL-9 when compared to cough group. Also, it showed elevated serum level of IL-9 compared to the other two phenotypes among individuals with IL4RA 175V AA and GG genotypes. SOB group showed higher prevalence of TT genotype of SNP IL-4C 590T in comparison to the cough group.
Conclusions: Our data show diversity in genotyping and cytokine profiles between asthma as a group and the proposed clinical phenotypes. This diversity clarifies the importance of classifying asthma depending on the proposed symptomatology.