alexa Six Cases of Infectious Mononucleosis by Cytomegalovirus as Diagnosed by Multiplex Virus PCR Assay | OMICS International | Abstract
ISSN: 2165-7831

Journal of Blood & Lymph
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Research Article

Six Cases of Infectious Mononucleosis by Cytomegalovirus as Diagnosed by Multiplex Virus PCR Assay

Nagao M1, Yoshioka Y1, Saito T1, Tsunemine H2, Ito K2, Kodaka T2, Tsuji G3, Watanabe K4, Shimizu N5* and Takahashi T2*

1Departments of Laboratory of Cell Therapy, Tokyo Medical and Dental University, Japan

2Department of Hematology, Shinko Hospital, Tokyo Medical and Dental University, Japan

3Departments of Rheumatology, Shinko Hospital, Kobe, Tokyo Medical and Dental University, Japan

4Departments of Pediatrics and Developmental Biology, Tokyo Medical and Dental University, Japan

5Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan

*Corresponding Author:
Takahashi T
Department of Hematology
Shinko Hospital, 1-4-47, Wakinohamacho
Chuo-ku, Kobe 651-0072, and Norio Shimizu
Center for Stem Cell and Regenerative Medicine
Tokyo Medical and Dental University
2-3-10 Kanda-Surugadai Chiyoda-ku
Tokyo 101-0062, Japan
Tel: 81- 78-261-6711
Fax: 81-78-261-6726
E-mail: [email protected]
Shimizu N
Department of Hematology
Shinko Hospital, 1-4-47, Wakinohamacho
Chuo-ku, Kobe 651-0072, and Norio Shimizu
Center for Stem Cell and Regenerative Medicine
Tokyo Medical and Dental University
2-3-10 Kanda-Surugadai Chiyoda-ku
Tokyo 101-0062, Japan
Tel: 81- 78-261-6711
Fax: 81-78-261-6726
E-mail: [email protected]

Received Date: April 20, 2017; Accepted Date: May 10, 2017; Published Date: May 17, 2017

Citation: Nagao M, Yoshioka Y, Saito T, Tsunemine H, Ito K, et al. (2017) Six Cases of Infectious Mononucleosis by Cytomegalovirus as Diagnosed by Multiplex Virus PCR Assay. J Blood Lymph 7: 165. doi: 10.4172/2165-7831.1000166

Copyright: © 2017 Nagao M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Infectious mononucleosis (IM) is mostly caused by Epstein-Barr virus (EBV) while IM by cytomegalovirus (CMV) is rather rare. In our retrospective clinical research with multiplex virus PCR analysis, we encountered 6 cases of CMV-IM and analyzed the clinical characteristics including diagnostic problems. The diagnosis of CMV-IM was made when the CMV genome was solely detected from the patient’s peripheral blood by multiplex virus PCR analysis. Then viral load was determined by quantitative PCR. Viral serological examinations were performed by a laboratory company as routine laboratory tests. Specific PCR signal for CMV genome was obtained by multiplex virus PCR in 6 patients, and blood CMV load ranged from 102 to 104 copies/mL. Clinical pictures and laboratory findings of these patients with CMV-IM were similar to those of EBV-IM in terms of fever, fatigue, morphology and number of atypical lymphocyte, and liver dysfunction. On serological examination, an IgM antibody against CMV was positive in all 6 patients; however, a VCA-IgM antibody against EBV was also positive in all patients examined, compromising serological differential diagnosis of IM. To make an exact diagnosis of CMV-IM, direct detection of the virus genome is important, and our multiplex virus PCR assay may be very useful in terms of quick performance and good specificity

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