Size-Exclusion Chromatography and Its Optimization for Material ScienceNetopilík M* and Trhlíková O
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic
- *Corresponding Author:
- Milos Netopilík
Institute of Macromolecular Chemistry
Academy of Sciences of the Czech Republic
Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic
Tel: +420 296809 295
Fax: +420 296809 410
E-mail: [email protected]
Received date: June 29, 2017; Accepted date: July 05, 2017; Published date: July 15, 2017
Citation: Netopilík M, Trhlíková O (2017) Size-Exclusion Chromatography and Its Optimization for Material Science. J Material Sci Eng 6: 358. doi: 10.4172/2169-0022.1000358
Copyright: © 2017 Netopilík M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, judged by statistical properties of the curves, both skew and excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction. In analyses of polystyrene standards by size exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the longitudinal diffusion is not important even at low flow-rates.