alexa Solid Phase Microextraction (SPME) Method Development in Analysis of Volatile Organic Compounds (VOCS) as Potential Biomarkers of Cancer | OMICS International | Abstract
ISSN-2155-9929

Journal of Molecular Biomarkers & Diagnosis
Open Access

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Review Article

Solid Phase Microextraction (SPME) Method Development in Analysis of Volatile Organic Compounds (VOCS) as Potential Biomarkers of Cancer

Kamila Schmidt* and Ian Podmore

Biomedical Science Research Centre, School of Environment and Life Sciences, University of Salford, M5 4WT, Manchester, UK

*Corresponding Author:
Kamila Schmidt
Biomedical Science Research Centre
School of Environment and Life Sciences
University of Salford, M5 4WT, UK
Tel: 0044 (0) 161 295 4649
E-mail: [email protected]

Received Date: September 29, 2015; Accepted Date: October 18, 2015; Published Date: October 21, 2015

Citation:Schmidt K, Podmore I (2015) Solid Phase Microextraction (Spme) Method Development in Analysis of Volatile Organic Compounds (Vocs) as Potential Biomarkers of Cancer. J Mol Biomark Diagn 6:253. doi:10.4172/2155-9929.1000253

Copyright: ©2015 Schmidt K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The analysis of volatile organic compounds [VOCs] is an attractive approach to the discovery of potential cancer biomarkers due to its non-invasive nature and potential low costs of sampling and analysis. Solid phase microextraction [SPME] is one of the main extraction techniques used to date for the collection of VOCs from both in vivo and in vitro samples in studies of potential biomarkers of various types of cancer. It offers simplicity of use, compatibility with both gas-chromatography [GC] and liquid-chromatography [LC] separation techniques and relatively lower costs. Development of the SPME method includes several important considerations: selection of the sampling mode, type of fiber and holder, optimisation of incubation, extraction and desorption conditions, and finally the use of an appropriate calibration procedure. This review summarizes and discusses the particular parameters of the SPME method development used by researchers to date for VOCs collection, from various biological matrices, in search of potential biomarkers of cancer.

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