alexa Specific TaqMan Probes for the Identification and Quantification of Lactobacilli in Pharmaceuticals | OMICS International
ISSN: 2329-8901

Journal of Probiotics & Health
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Research Article

Specific TaqMan Probes for the Identification and Quantification of Lactobacilli in Pharmaceuticals

Herbel SR1,2*, Von Nickisch-Rosenegk M3, Kuhn M4, Murugaiyan J5, Wieler LH1 and Guenther S1
1Freie Universität Berlin, Centre for Infection Medicine, Institute of Microbiology and Epizootics, Robert-von-Ostertag--Str.7-13, 14163 Berlin, Germany
2Freie Universität Berlin, Department of Biology, Chemistry, Pharmacy, Takustr. 3, 14195 Berlin, Germany
3Fraunhofer Institute, IBMT Potsdam-Golm, Am Mühlenberg 13, 14476 Potsdam, Germany
4CONGEN Biotechnologie GmbH, Robert-Rössle-Straße 10, 13125 Berlin, Germany
5Freie Universität Berlin, Centre for Infection Medicine, Institute of Animal and Environmental Hygiene, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany
Corresponding Author : Herbel SR
Freie Universität Berlin, Centre for Infection Medicine, Institute of Microbiology and Epizootics
Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany
Tel: 00493083851901
E-mail: [email protected]
Received December 20, 2013; Accepted January 28, 2014; Published February 04, 2014
Citation: Herbel SR, Von Nickisch-Rosenegk MV, Kuhn M, Murugaiyan J, Wieler LH, et al. (2014) Specific TaqMan® Probes for the Identification and Quantification of Lactobacilli in Pharmaceuticals. J Prob Health 2:115. doi:10.4172/2329-8901.1000115
Copyright: © 2014 Herbel SR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Several probiotic products containing species of the genus Lactobacillus are available on the market. Usually, these are fermented dairy products such as yoghurts and vegetables. Additionally, probiotic bacteria are used in pharmaceuticals, which are also believed to have beneficial effects on human health. Common pharmaceutical application forms to deliver probiotics are tablets, drops or granulate formulations for oral administration. They should contain sufficient numbers of viable probiotics to assure active health benefits. Despite the successful commercialization of lactobacilli, their traditional species identification methods are time-consuming and laborintensive and do not allow quantification of the species. Therefore, the objective of the present work was to develop a culture independent, fast identification and quantification method for two commercially important species of the genus Lactobacillus (L. acidophilus and L. reuteri). We used a TaqMan® real time PCR assay based on the GroEL heat shock protein region. Therefore universal lactobacilli primers and species-specific TaqMan® primers have been developed. The assay allowed an unambiguous species-specific detection of L. acidophilus and L. reuteri from bacterial cultures as well as directly from tablets. Using this assay, we were able to detect lactobacilli strains to a level of 104 cfu/ml, which is a sufficient detection limit as commercial pharmaceuticals usually contain 108–1010 cfu/ tablet of probiotic strains.

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