alexa Spectrofluorimetric Estimation of Some SulfhydrylâÂ
ISSN : 2153-2435

Pharmaceutica Analytica Acta
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Research Article

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Haggag RS1,2*, Belal SF1, Hewala II1 and ElRouby OA3

1Pharmaceutical Analytical Chemistry Department, University of Alexandria, Alexandria, Egypt

2Analytical and Pharmaceutical Chemistry Department, Pharos University in Alexandria, Alexandria, Egypt

3European Egyptian Company for Pharmaceutical industries, Alexandria, Egypt

*Corresponding Author:
Haggag RS
Pharmaceutical Analytical Chemistry Department
Faculty of Pharmacy, University of Alexandria
Elmessalah 21521, Alexandria, Egypt
Tel: +20-3-487-1317
Fax: + 20-3-487-3273
E-mail: [email protected]

Received Date: November 04, 2016; Accepted Date: January 09, 2017; Published Date: January 13, 2017

Citation: Haggag RS, Belal SF, Hewala II, ElRouby OA (2017) Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid. Pharm Anal Acta 8:532. doi: 10.4172/2153-2435.1000532

Copyright: © 2017 Haggag RS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A simple and sensitive spectrofluorimetric method has been developed for the determination of some selected sulfhydryl–containing drugs namely Acetylcysteine (ACS), Captopril (CAP) and Mesna (MSN). The method is based on the interaction of the drugs with potassium (5-sulfoxino) palladium II in alkaline medium in presence of magnesium ions, where the sulfhydryl group combines with palladium from the non-fluorescent potassium bis (5-sulfoxino) palladium II. The resulting 8-hydroxy-5-quinoline sulfonic acid coordinates with magnesium to form the fluorescent chelate that is a measure of the amount of sulfhydrl containing drug analyzed. The fluorescence intensity was measured at an emission wavelength of 485 nm, by excitation at 345 nm. All the experimental parameters affecting the reaction were studied and optimized. The proposed method was applicable over the concentration range of 0.04-0.44 μg/mL for the three drugs and was applied for their determination in bulk form and in pharmaceutical preparations without interference from common excipients. The assay results were statistically compared with those obtained from previously reported methods where no significant difference was found between them. The selectivity and the stability-indicating aspect of the proposed method were confirmed by preparing the disulphides of the studied drugs and applying the reaction to the parent drugs in presence of their disulphides where no interference was detected from these related substances. By virtue of its high sensitivity, the proposed method was also extended to analyze the drugs in spiked human plasma and urine.

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