Spectrophotometric Determination and Commercial Formulation of Tebuconazole Fungicide after Derivatization
Kafeel Ahmad Khan* and Farzana Haider
Bacha Khan University, Charsadda, KPK, Pakistan
- *Corresponding Author:
- Kafeel Ahmad Khan
Bacha Khan University, Charsadda
24420, KPK, Pakistan
E-mail: [email protected]
Received Date: June 10, 2016; Accepted Date: June 26, 2016 ; Published Date: June 30, 2016
Citation: Khan KA, Haider F (2016) Spectrophotometric Determination and Commercial Formulation of Tebuconazole Fungicide after Derivatization. Chem Sci J 7:133. doi:10.4172/2150-3494.1000133
Copyright: © 2016 Khan KA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A spectrophotometric method has been developed for the determination of tebuconazole fungicide formulation. The method is based on the complexation reaction of ferric chloride with tebuconazole fungicide in acidic media and the purple colored complex was studied at 530 nm. The reaction conditions and other analytical parameters were optimized. A linear calibration curve between absorbance and concentration over the range from 0.2 to 20 μg mL−1 was obtained with molar absorptivity of 2.1 × 104 L mol−1 cm−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.05 and 0.2 μg mL−1 respectively. The proposed method has been successfully applied for the analysis of commercial formulation of tebuconazole fungicide. The recoveries of the method were found to be in the range of 92.16 ± 0.06% to 96.66 ± 0.18%.