Spectrophotometric Determination of Nicorandil in Bulk and Pharmaceutical Formulation Using Phospho-Molybdenum Blue Complex
- *Corresponding Author:
- Nariman M Saeed
Ministry of Health, Egypt
Tel: (20-2) 7951821 / 794
E-mail: [email protected]
Received Date: May 03, 2017; Accepted Date: May 18, 2017; Published Date: May 25, 2017
Citation: El-Adl SM, El-Sadek ME, Saeed NM (2017) Spectrophotometric Determination of Nicorandil in Bulk and Pharmaceutical Formulation Using Phospho-Molybdenum Blue Complex. J Appl Pharm 9:243. doi: 10.21065/1920-4159.1000243
Copyright: © 2017 El-Adl SM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A new spectrophotometric method is described for the determination of nicorandil in bulk and pharmaceutical dosage form. It is based on the reduction of nitrate in nicorandil to nitrite ion by using vanadium chloride, and reduction of phosphomolybdic acid to phospho-molybdenum blue complex by sodium sulfide, phospho-molybdenum blue complex is then oxidized by nitrite ion leading to a decrease in blue color intensity which found to be directly proportional to the concentration of nicorandil. Maximum absorbance was measured at 827 nm. Effect of acidity, volume of Sodium Sulfide, stability of complex, volume of vanadium chloride, time and temperature of the reaction were completely studied. The proposed method was satisfactorily applied for the determination of the drug in both bulk and pharmaceutical forms, the calibration curve was linear over the range (60–200 μg/ml) and the results were compared statistically with reference methods.