Spectroscopic Investigation on the Interaction of Pb(II) with Keyhole Limpet Hemocyanin
Yulan Fan, Huifang Chen, Guidi Zeng, Jingyi Liu, Jun Xue, Yongquan Wu and Xun Li*
School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou Jiangxi, PR China
- *Corresponding Author:
- Xun Li
School of Chemistry and Chemical Engineering
Gannan Normal University, Ganzhou Jiangxi, 341000, PR China
E-mail: [email protected]
Received date: June 02, 2016; Accepted date: June 13, 2016; Published date: June 17, 2016
Citation: Fan Y, Chen H, Zeng G, Liu J, Xue J, et al. (2016) Spectroscopic Investigation on the Interaction of Pb(II) with Keyhole Limpet Hemocyanin. Med chem (Los Angeles) 6:395-398. doi:10.4172/2161-0444.1000375
Copyright: © 2016 Fan Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The interaction between Pb2+ and Keyhole Limpet Hemocyanin (KLH) was investigated by fluorescence, Ultraviolet-visible (UV-Vis) absorption and Circular Dichroism (CD) spectroscopy. The experimental results showed that Pb2+ could quench the intrinsic fluorescence of KLH following static and dynamic quenching process. The number of binding sites n was approximately equal to 1. ΔH<0 and ΔS<0 indicated that a Pb-KLH complex was formed. Furthermore, the data of synchronous fluorescence, UV-Vis and CD spectra suggested that Pb2+ changed the micro-environment and conformation of KLH.