Spermatocyte Spreading during Meiotic Cell Preparation is a Two Step ProcessRazan Muhtadi1, Emad A. Ahmed1,2 and Harry Scherthan1*
- *Corresponding Author:
- Harry Scherthan
Inst. für Radiobiologie der Bundeswehr in Verb. mit der Univ. Ulm
D-80937 Munich, Germany
Tel: +49 89 992 692 2272
Fax: +49 89 992 692 2255
E-mail: [email protected]
Received Date: Mar 20, 2017; Accepted Date: Apr 14, 2017; Published Date: Apr 26, 2017
Citation: Muhtadi R, Ahmed EA, Scherthan H (2017) Spermatocyte Spreading during Meiotic Cell Preparation is a Two Step Process . J Cytol Histol 8: 1000450. doi:10.4172/2157-7099.1000450
Copyright: © 2017 Muhtadi, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cytological techniques have been instrumental for the investigation of meiosis and gametogenesis. Especially high resolution chromatin spreads of male and female germline cells provide for detailed insights into the molecular mechanisms acting during germ cell differentiation. Most spreading techniques for germ cells are done with a hypotonic detergent solution followed by an extended drying period. Using video microscopy and we monitored the course of mouse spermatocyte spreading after exposure to graded concentrations of the ionic detergent mixture Lipsol that is known to efficiently spread meiocytes. Our analysis disclosed that spreading of meiotic cells is optimal at a final detergent concentration of about 0.7% and occurs in two phases. First, cells undergo slow swelling in the dilute detergent solution (lasting up to >1 h) followed by rapid dispersion of the nuclear chromatin over the glass surface just prior to the final evaporation of the water in the solution. These results provide a better understanding of an important technique in meiosis research and identify factors determining the spreading process.