Spontaneous Chondrocyte Maturation on 3D-Chitosan Scaffolds
- Corresponding Author:
- Cristina C Teixeira, DMD, MS, PhD
Department of Basic Science and Craniofacial Biology
New York University College of Dentistry
345 East 24th Street
New York, NY 10010, USA
Tel: +(212) 998- 9958
E-mail: [email protected]
Received date: January 03, 2013; Accepted date: February 12, 2013; Published date: February 15, 2013
Citation: Oliveira SM, Turner G, Rodrigues SP, Barbosa MA, Alikhani M, et al. (2013) Spontaneous Chondrocyte Maturation on 3D-Chitosan Scaffolds. J Tissue Sci Eng 4:124. doi:10.4172/2157-7552.1000124
Copyright: © 2013 Oliveira SM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The endochondral ossification mechanism provides promising new insight into potential techniques for repairing bone defects. The methods for replicating endochondral ossification in culture, although diverse, have yet to identify a procedure that reflects natural conditions, while satisfying the practical, experimental needs for efficiency and maneuverability. In this study, we present a novel approach that addresses all of these concerns. Coordinated proliferation and differentiation of chondrocytes in a transient cartilage is required for endochondral bone growth, but the mechanisms and pathways that control these processes are not completely understood. For lack of alternative methods, chondrocyte cultures are commonly pushed to differentiation with growth factors and supplements, like retinoic acid. We have developed a tissue culture model, using a 3D-chitosan sponge, which supports the proliferation and differentiation of chondrocytes, without exposure to supplements, and which matures into a near replica of the growth plate.