Spontaneous One-Lung Ventilation Increases the Lung Inflammatory Response: An Experimental Pilot StudyHumberto S Machado1*, Paula Sá1, Catarina S Nunes2, António Couceiro3, Álvaro Moreira da Silva4 and Artur Águas5
- *Corresponding Author:
- Humberto S Machado
Department, Centro Hospitalar do Porto
Largo Abel Salazar, 4099-001 Porto, Portugal
Tel: 351 935848475
Fax: 351 220900644
E-mail: [email protected]
Received date: May 28, 2014; Accepted date: August 01, 2014; Published date: August 11, 2014
Citation: Machado HS, Sá P, Nunes CS, Couceiro A, da Silva ÁM, et al. (2014) Spontaneous One-Lung Ventilation Increases the Lung Inflammatory Response: An Experimental Pilot Study. J Anesth Clin Res 5:428. doi: 10.4172/2155-6148.1000428
Copyright: © 2014 Machado HS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Study objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure.
Design: In vivo prospective randomised animal study
Setting: University research laboratory
Subjects: New Zealand rabbits Interventions: Rabbits (n=20) were randomly assigned to 4 groups (n=5 each group). Groups 1 and 2 were submitted to one-lung ventilation, during 20 and 75 minutes respectively; groups 3 and 4 were submitted to two-lung ventilation during 20 and 75 minutes and considered controls. Ketamine/xylazine was administered for induction and maintenance of anesthesia. One-lung ventilation was achieved by administration of air into the interpleural space, and left lung collapse was visually confirmed through the centre of the diaphragm.
Measurements: Lung histology preparations were observed under light microscopy for quantification of the inflammatory response (light, moderate and severe).
Main results: All subjects had at least light inflammatory response. However, rabbits submitted to one-lung ventilation had a statistically significant value for the occurrence of moderate inflammation (p<0.05). The inflammatory response found included mainly eosinophils, with an average proportion of 75/25 to other polymorphonuclear cells. No differences between groups were found regarding gas exchange, heart rate and respiratory rate.
Conclusions: In this spontaneous one-lung ventilation model, lung collapse was positively associated with a greater inflammatory response when compared to normal two-lung ventilation.