Spontaneous One-Lung Ventilation Increases the Lung Inflammatory Response: An Experimental Pilot Study
|Humberto S Machado1*, Paula Sá1, Catarina S Nunes2, António Couceiro3, Álvaro Moreira da Silva4 and
|1Anesthesiology Department, Centro Hospitalar do Porto, Largo Abel Salazar, 4099-001 Porto, Portugal|
|2Open University, Department of Science and Technology and Anesthesiology Department, Centro Hospitalar do Porto, Largo Abel Salazar, 4099-001 Porto, Portugal|
|3Pathological Anatomy service, Gaia/Espinho Hospital Centre, Rua Conceição Fernandes, 4430 Vila Nova de Gaia, Portugal|
|4Intensive care Service, Port Hospital Center, Largo Abel Salazar, 4099-001 Porto, Portugal|
|5Department of Normal Anatomy, Abel Salazar Biomedical Sciences Institute-University of Porto and Multidisciplinary Biomedical Research Unit, St. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal|
|Corresponding Author :||Humberto S Machado
Department, Centro Hospitalar do Porto
Largo Abel Salazar, 4099-001 Porto, Portugal
Tel: 351 935848475
Fax: 351 220900644
E-mail: [email protected]
|Received May 28, 2014; Accepted August 01, 2014; Published August 11, 2014|
|Citation: Machado HS, Sá P, Nunes CS, Couceiro A, da Silva ÁM, et al. (2014) Spontaneous One-Lung Ventilation Increases the Lung Inflammatory Response: An Experimental Pilot Study. J Anesth Clin Res 5:428. doi: 10.4172/2155-6148.1000428|
|Copyright: © 2014 Machado HS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Study objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure.
Design: In vivo prospective randomised animal study
Setting: University research laboratory
Subjects: New Zealand rabbits Interventions: Rabbits (n=20) were randomly assigned to 4 groups (n=5 each group). Groups 1 and 2 were submitted to one-lung ventilation, during 20 and 75 minutes respectively; groups 3 and 4 were submitted to two-lung ventilation during 20 and 75 minutes and considered controls. Ketamine/xylazine was administered for induction and maintenance of anesthesia. One-lung ventilation was achieved by administration of air into the interpleural space, and left lung collapse was visually confirmed through the centre of the diaphragm.
Measurements: Lung histology preparations were observed under light microscopy for quantification of the inflammatory response (light, moderate and severe).
Main results: All subjects had at least light inflammatory response. However, rabbits submitted to one-lung ventilation had a statistically significant value for the occurrence of moderate inflammation (p<0.05). The inflammatory response found included mainly eosinophils, with an average proportion of 75/25 to other polymorphonuclear cells. No differences between groups were found regarding gas exchange, heart rate and respiratory rate.
Conclusions: In this spontaneous one-lung ventilation model, lung collapse was positively associated with a greater inflammatory response when compared to normal two-lung ventilation.