Squamous Cell Atypia Suggesting High-grade Lesions: Correlation of Morphological Data, HPV Test Results and Follow-upAlameda F1,2*, Hernández E1, Del Amo E1,2, Bellosillo B1,3, Serrano L1 and Carreras R1,2
- *Corresponding Author:
- Alameda F
Department of Pathology
c/Passeig Maritim 25-2908003 Barcelona, Spain
E-mail: [email protected]
Received Date: Jan 19, 2016; Accepted Date: Mar 08, 2016; Published Date: Mar 18, 2016
Citation: Hernández E, Del Amo E, Bellosillo B, Serrano L, Alameda F, et al. (2016) Squamous Cell Atypia Suggesting High-grade Lesions: Correlation of Morphological Data, HPV Test Results and Follow-up. J Cytol Histol 7:396. doi:10.4172/2157-7099.1000396
Copyright: © 2016 Alameda F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Squamous cell atypia suggesting high-grade lesion (ASCH) is a cytological lesion, related to a high-grade lesion. Guidelines recommend performing a colposcopy in these cases, without HPV testing.
Objective: Here we sought to determine the value of the HPV test in ASCH cases and to correlate morphological data, the HPV test results, and the follow-up.
Methods: Ninety-six patients were diagnosed with ASCH using liquid-based cytology (Thin-Prep®). Hybrid Capture II was the HPV test used. The patients were followed up for 6 to 48 months (mean 24 months). The following cytological data were included: isolated cells; dyskeratosis; hyperchromasia; coarse chromatin; nuclear membrane irregularity; 3D alterations; and molding.
Results: The mean age of the patients was 37 years (range 15-78). Thirty-seven cases were 30 years old or younger. Follow-up was not available for 11 cases. Seventy two cases were HPV+. None of HPV- cases developed HSIL. The following cytological data showed statistical significance: coarse chromatin and HPV positivity (p = 0.007); nuclear membrane irregularity and HSIL in the follow-up (p = 0.015); and molding and HSIL in the follow-up in HPV+ cases (p = 0.046).
Conclusions: The HPV test is useful for ASCH cases. Some nuclear alterations in ASCH can predict HPV positivity and HSIL in the follow-up.