alexa Stability Indicating HPLC Method for the Simultaneous D
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Stability Indicating HPLC Method for the Simultaneous Determination of Ceftriaxone and Vancomycin in Pharmaceutical Formulation

Gurupadayya BM* and Disha NS

Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS University, Mysore, Karnataka, India

*Corresponding Author:
Gurupadayya BM
Department of Pharmaceutical Analysis
JSS College of Pharmacy, JSS University
Mysore–570 015, Karnataka, India
Tel: 917411006136
E-mail: [email protected]

Received Date: November 10, 2013; Accepted Date: November 28, 2013; Published Date: November 30, 2013

Citation: Gurupadayya BM, Disha NS (2013) Stability Indicating HPLC Method for the Simultaneous Determination of Ceftriaxone and Vancomycin in Pharmaceutical Formulation. J Chromatograph Separat Techniq 4:207. doi:10.4172/2157-7064.1000207

Copyright: © 2013 Gurupadayya BM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A simple, sensitive, rapid, robust and reproducible method for the simultaneous determination of ceftriaxone and vancomycin in formulation was developed using reverse phase high performance liquid chromatographic method. The analysis was performed on C8 (250×4.6 mm, 5 μm) column with a mobile phase consisting of 0.01 M of potassium di hydrogen ortho phosphate and 0.01M of disodium hydrogen phosphate buffer (pH 4.2), methanol in the ratio of 70:30 (v/v) with a flow rate of 1ml/min. The analyte was examined with UV detector at 298 nm. In the developed method vancomycin elutes at 2.7 min and ceftriaxone at 3.7 min. The proposed method is having linearity in the concentration range from 20-100 μg/ml of ceftriaxone and 10-50 μg/ml vancomycin. The method was validated with respect to system suitability, precision, linearity, limit of detection (LOD) and limit of quantification (LOQ), accuracy, recovery, robustness, stability. The proposed method can be readily utilized for determination of ceftriaxone and vancomycin.


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