alexa Stability Indicating LC Method for the Estimation of Be
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Stability Indicating LC Method for the Estimation of Benazepril HCl and Hydrochlorthiazide in Pharmaceutical Dosage Form

Chhalotiya UK*, Varsha LP, Dimal AS, Kashyap KB and Sunil LB

Department of Pharmaceutical Analysis, Indukaka Ipcowala College of Pharmacy, Gujarat, India

*Corresponding Author:
Chhalotiya UK
Indukaka Ipcowala College of Pharmacy
Beyond GIDC Phase IV, Vithal Udyognagar
New Vallabh Vidyanagar -388121, Anand, Gujarat, India
Tel: 919924712908
E-mail: [email protected]

Received date: January 31, 2014; Accepted date: March 26, 2014; Published date: March 30, 2014

Citation: Chhalotiya UK, Varsha LP, Dimal AS, Kashyap KB, Sunil LB (2014) Stability Indicating LC Method for the Estimation of Benazepril HCl and Hydrochlorthiazide in Pharmaceutical Dosage Form. J Chromatograph Separat Techniq 5:216. doi:10.4172/2157-7064.1000216

Copyright: © 2014 Chhalotiya UK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A rapid, specific and sensitive reverse phase high performance liquid chromatographic method has been developed and validated for analysis of benazepril hydrochloride and hydrochlorothiazide in both bulk and pharmaceutical dosage form. A sunfire C-18, 250×4.6 mm i.d. and 5 μm particle size column with mobile phase containing water: methanol (55:45, v/v, pH 7). The flow rate was 1.0 mL min-1 and effluents were monitored at 233 nm. The retention time of benazepril hydrochloride and Hydrochlorthiazide was 9.19 min and 3.10 min respectively. Benazepril hydrochloride and hydrochlorthiazide was subjected to acid and alkali hydrolysis, chemical oxidation, wet hydrolysis, dry heat degradation and sun light degradation. The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values. Stressed samples were assayed using developed LC method. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of benazepril hydrochloride and hydrochlorthiazide in tablet dosage forms.


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