alexa Stability-indicating HPLC Method for the Determination
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Stability-indicating HPLC Method for the Determination of Atenolol in Pharmaceutical Preparations

Belal F1, Sharaf El-Din M1, Aly F2, Hefnawy M3 and El-Awady M1*
1Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt
2Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia
3Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Corresponding Author : M. El-Awady
Department of Analytical Chemistry
Faculty of Pharmacy
University of Mansoura
Mansoura 35516, Egypt
Tel: 20-50- 2247496
E-mail: [email protected]
Received January 12, 2013; Accepted January 30, 2013; Published February 04, 2013
Citation: Belal F, Sharaf El-Din M, Aly F, Hefnawy M, El-Awady M (2013) Stability-indicating HPLC Method for the Determination of Atenolol in Pharmaceutical Preparations. J Chromat Separation Techniq 4:164. doi:10.4172/2157-7064.1000164
Copyright: © 2013 Belal F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

A stability-indicating reversed-phase liquid chromatographic method for the determination of atenolol was developed. Different chromatographic conditions were carefully studied to optimize the parameters for the evaluation of the studied drug. The chromatographic assay involved the use of a C8 Column (250 mm×4.6 mm i.d., 5 μm) with a simple mobile phase composed of acetonitrile:methanol:0.02 M phosphate buffer, pH 5 (20:20:60) at a flow rate of 1 ml/min and UV detection at 226 nm. Pindolol was used as an internal standard. The method showed good linearity over the range of 0.05-10 μg/mL with a detection limit of 0.01 μg/mL and a quantitation limit of 0.03 μg/mL. The proposed method was successfully applied for the analysis of atenolol in three commercial tablets with average percent recoveries of 100.14 ± 1.04, 100.20 ± 0.92, 100.00 ± 0.91 and 100.75 ± 0.67, respectively. Several co-formulated and co-administered drugs did not interfere with the proposed method. The results were statistically compared with those obtained by the official method and were found to be in good agreement. The stability-indicating capability of the method was also tested after accelerated degradation of atenolol in acidic and basic media and after freezing and heating treatments.

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