alexa Stable Expression of Anti-CD52 Monoclonal Antibody Using a Bicistronic Vector System
ISSN: 0974-8369

Biology and Medicine
Open Access

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Research Article

Stable Expression of Anti-CD52 Monoclonal Antibody Using a Bicistronic Vector System

Azam Rahimpour1,2*, Hadi Bayat1,2, Meysam Omidi1,2, Morvarid Peyrovan3, Omid Mohammadian4 and Fatemeh Naderi5

1Medical Nano-Technology & Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Deaprtment of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

4Departments of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5Department of Genetics, School of Advanced Sciences, Islamic Azad University, Tehran Medical Branch, Tehran, Iran

*Corresponding Author:
Rahimpour A
School of Advanced Technologies in Medicine, Taleghani Educational Hospital
Araabi St., Yaman Ave, Chamran High Way
Tehran 1985711151, Iran
E-mail: rahimpour.az@gmail.com

Received Date: August 14, 2016; Accepted Date: September 15, 2016; Published Date: September 22, 2016

Citation: Rahimpour A, Bayat H, Omidi M, Peyrovan M, Mohammadian O, et al. (2016) Stable Expression of Anti-CD52 Monoclonal Antibody Using a Bicistronic Vector System. Biol Med (Aligarh) 8: 341. doi:10.4172/0974-8369.1000341

Copyright: © 2016 Bayat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The efficient development of stable monoclonal antibody-producing mammalian cells is a tedious and time-consuming process due to the structural complexity of these molecules. The ratio of the light-chain to heavy-chain expression is critical for the assembly and successful production of functional antibodies. Different vector-design strategies have been employed for the optimal expression of monoclonal antibodies in mammalian cells. In the current study, a bicistronic expression based on the encephalomyocarditis virus internal ribosomal entry site (ECMV IRES) element was used for the development of Chinese hamster ovary (CHO)–stable cell pools expressing an anti-CD52 antibody. The successful expression of the monoclonal antibody in CHO cells was achieved with the maximum titer of 20 μg/l. Our results here show that IRES-mediated bicistronic expression is an efficient method for the stable expression of monoclonal antibodies in CHO cells.

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